Publication: Micropropagation of Rhynchostylis gigantea var. rubra through protocorm-like bodies
Issued Date
2014-03-13
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05677572
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2-s2.0-84899538023
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Mahidol University
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SCOPUS
Bibliographic Citation
Acta Horticulturae. Vol.1025, (2014), 215-222
Suggested Citation
S. Prasongsom, K. Thammasiri, N. Chuenboonngarm, W. Noonpakdee Micropropagation of Rhynchostylis gigantea var. rubra through protocorm-like bodies. Acta Horticulturae. Vol.1025, (2014), 215-222. doi:10.17660/ActaHortic.2014.1025.31 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33038
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Title
Micropropagation of Rhynchostylis gigantea var. rubra through protocorm-like bodies
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Abstract
Rhynchostylis gigantea var. rubra is a variety of purple-red flower orchid. There is small amount of this orchid in natural habitat. Shoot-tip culture was applied for asexual propagation to maintain its good characteristics. Efficient methods for micropropagation through protocorm-like bodies (PLBs) from shoottips using various plant tissue culture media and sucrose concentrations were studied. After two months of culture, the highest survival rate of 92.6% and the highest PLB formation (7.9 PLBs) were found in a modified Vacin and Went liquid medium with 1% (w/v) sucrose. Shoot proliferation was effectively induced by using a basal Schenk and Hildebrandt (SH). PLBs cultured in SH media supplemented with 0.1 mg L-1thidiazuron (TDZ) produced a high number of shoots/PLB but had no root while those cultured in PGR free SH media or SH media supplemented with 0.5 mg L-1NAA produced lots of shoots with roots.