Publication: New facet of non-o1/non-o139 vibrio cholerae hemolysin a: A competitive factor in the ecological niche
Issued Date
2018-01-01
Resource Type
ISSN
15746941
01686496
01686496
Other identifier(s)
2-s2.0-85040638932
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
FEMS Microbiology Ecology. Vol.94, No.1 (2018)
Suggested Citation
Pichet Ruenchit, Onrapak Reamtong, Kanokrat Siripanichgon, Wanpen Chaicumpa, Pornphan Diraphat New facet of non-o1/non-o139 vibrio cholerae hemolysin a: A competitive factor in the ecological niche. FEMS Microbiology Ecology. Vol.94, No.1 (2018). doi:10.1093/femsec/fix113 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45898
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
New facet of non-o1/non-o139 vibrio cholerae hemolysin a: A competitive factor in the ecological niche
Other Contributor(s)
Abstract
© FEMS 2017. Different serogroups of Vibrio cholerae may inhabit the same ecological niche. However, serogroup O1/O139 strains are rarely isolated from their ecological sources. Quite plausibly, the non-O1/non-O139 vibrios and other bacterial species suppress growth of O1/O139 strains that share the same niche. Our bacterial inhibition assay data indicated that certain non-O1/non-O139 strains used a contact-dependent type VI secretion system (T6SS) to suppress growth of the O1 El Tor, N16961 pandemic strain. Comparative proteomics of the O1 and the suppressive non-O1/non-O139 strains co-cultured in a simulated natural aquatic microcosm showed that SecB and HlyD were upregulated in the latter. The HlyD-related effective factor was subsequently found to be hemolysin A (HlyA). However, not all hlyA-positive non-O1/non-O139 strains mediated growth suppression of the N16961 V. cholerae; only strains harboring intact cluster I HlyA could exert this activity. The key feature of the HlyA is located in the ricin-like lectin domain (β-trefoil) that plays an important role in target cell binding. In conclusion, the results of this study indicated that non-O1/non-O139 V. cholerae suppressed the growth of the O1 pandemic strain by using contact-dependent T6SS as well as by secreting the O1-detrimental hemolysin A during their co-persistence in the aquatic habitat.