Publication: Essential role of tryptophan residues in toxicity of binary toxin from Bacillus sphaericus
Issued Date
2011-10-01
Resource Type
ISSN
1976670X
19766696
19766696
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2-s2.0-84857703761
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Mahidol University
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SCOPUS
Bibliographic Citation
BMB Reports. Vol.44, No.10 (2011), 674-679
Suggested Citation
Thittaya Kunthic, Boonhiang Promdonkoy, Toemsak Srikhirin, Panadda Boonserm Essential role of tryptophan residues in toxicity of binary toxin from Bacillus sphaericus. BMB Reports. Vol.44, No.10 (2011), 674-679. doi:10.5483/BMBRep.2011.44.10.674 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11460
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Title
Essential role of tryptophan residues in toxicity of binary toxin from Bacillus sphaericus
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Abstract
Bacillus sphaericus produces mosquito-larvicidal binary toxin composed of BinA and BinB. While BinB is expected to bind to a specific receptor on the cell membrane, BinA interacts to BinB or BinB receptor complex and translocates into the cytosol to exert its activity via unknown mechanism. To investigate functional roles of aromatic cluster in BinA, amino acids at positions Y213, Y214, Y215, W222 and W226 were substituted by leucine. All mutant proteins were highly produced and their secondary structures were not affected by these substitutions. All mutants are able to insert into lipid monolayers as observed by Langmuir-Blodgett trough and could permeabilize the liposomes in a similar manner as the wild type. However, mosquito-larvicidal activity was abolished for W222L and W226L mutants suggesting that tryptophan residues at both positions play an important role in the toxicity of BinA, possibly involved in the cytopathological process after toxin entry into the cells.