Publication: Comprehensive investigation of common antibody-dependent cell-mediated cytotoxicity antibody epitopes of HIV-1 CRF01-AE gp120
Issued Date
2012-10-01
Resource Type
ISSN
19318405
08892229
08892229
Other identifier(s)
2-s2.0-84866681537
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
AIDS Research and Human Retroviruses. Vol.28, No.10 (2012), 1250-1258
Suggested Citation
Silawun Ampol, Kovit Pattanapanyasat, Ruengpung Sutthent, Parichart Permpikul, Wannee Kantakamalakul Comprehensive investigation of common antibody-dependent cell-mediated cytotoxicity antibody epitopes of HIV-1 CRF01-AE gp120. AIDS Research and Human Retroviruses. Vol.28, No.10 (2012), 1250-1258. doi:10.1089/aid.2011.0346 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/14258
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Comprehensive investigation of common antibody-dependent cell-mediated cytotoxicity antibody epitopes of HIV-1 CRF01-AE gp120
Other Contributor(s)
Abstract
The antibody-dependent cell-mediated cytotoxicity (ADCC) mechanism involves both innate and adaptive immune systems. While a number of epitope mapping studies of neutralizing (Nt) antibodies and cytotoxic T lymphocyte (CTL) against a variety of HIV-1 clades have been reported, there has been a paucity of similar studies aimed at identifying epitopes of ADCC-inducing antibodies. Herein we screened 35 sera from HIV-1 CRF01-AE-infected blood donors for ADCC antibody activity against gp120 utilizing an EGFP-CEM-NK r flow cytometric assay. Eighteen sera with high ADCC antibody activity were then comprehensively examined for ADCC antibody epitopes using the HIV-1 subtype CRF01-AE TH023 gp120 peptide set consisting of 126 peptides of 15 amino acids in length, overlapping by 11 amino acids. This peptide set was divided into five pools (E1-E5). Each positive peptide pool was further investigated for fine epitope mapping of ADCC antibody activity using a 5 by 5 peptide matrix format. Interestingly, six and three peptides from peptide pools E1 and E2, respectively, responded to at least 33.33% of the tested sera. These nine common ADCC epitopes were localized to the C1 and V2 region of gp120. Furthermore, 5/9 epitopes were also shown to serve as full or partial Nt antibody targets for HIV-1 subtypes B and CRF01-AE. We submit these data on epitope mapping of ADCC or dual ADCC-Nt antibodies against HIV-1 gp120 that should be considered in the formulation of vaccines against HIV-1. © 2012, Mary Ann Liebert, Inc.