Publication: Multispecies detection of antibodies to influenza A viruses by a double-antigen sandwich ELISA
Issued Date
2010-02-01
Resource Type
ISSN
01660934
Other identifier(s)
2-s2.0-73049083540
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Virological Methods. Vol.163, No.2 (2010), 238-243
Suggested Citation
Kamolwan Watcharatanyatip, Sirikwan Boonmoh, Kridsada Chaichoun, Taweesak Songserm, Mingkhwan Woratanti, Tararaj Dharakul Multispecies detection of antibodies to influenza A viruses by a double-antigen sandwich ELISA. Journal of Virological Methods. Vol.163, No.2 (2010), 238-243. doi:10.1016/j.jviromet.2009.09.027 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/29271
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Multispecies detection of antibodies to influenza A viruses by a double-antigen sandwich ELISA
Other Contributor(s)
Abstract
A double-antigen sandwich ELISA was developed for the detection of antibodies to influenza A viruses. A recombinant nucleoprotein (rNP) of influenza A virus was used as a capture antigen and an HRP-conjugate for detecting the antibodies. A total of 125 serum samples from birds of different species including chickens, geese, open-billed storks, Khaki Campbell ducks, lesser whistling ducks, and pigeons with known antibodies were tested by ELISA. The sensitivity and the specificity of ELISA were found to be 98% and 97.3%, respectively. The assay was able to detect the presence of influenza A antibodies as early as the fourth day post-inoculation in ducks infected experimentally with influenza A (H5N1) virus. Excellent agreement (97.6%) was obtained between this sandwich ELISA and the hemagglutination inhibition (HI) tests (κ = 0.95). The double-antigen sandwich ELISA correlated well with a commercial avian influenza (AI) multispecies ELISA and was slightly more sensitive than the AI multispecies ELISA. These findings indicate that the double-antigen sandwich ELISA based on rNP may offer an effective screening method for serodiagnosis of influenza A virus. The double-antigen sandwich ELISA also enables the detection of antibodies to influenza A viruses in different species without the need for species-specific secondary antibodies. © 2009 Elsevier B.V. All rights reserved.