Publication: Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures
Issued Date
2010-05-01
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01676857
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2-s2.0-77952010989
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Mahidol University
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SCOPUS
Bibliographic Citation
Plant Cell, Tissue and Organ Culture. Vol.101, No.2 (2010), 119-126
Suggested Citation
Panitch Boonsnongcheep, Sirintra Korsangruang, Noppamas Soonthornchareonnon, Yupyn Chintapakorn, Promchit Saralamp, Sompop Prathanturarug Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures. Plant Cell, Tissue and Organ Culture. Vol.101, No.2 (2010), 119-126. doi:10.1007/s11240-010-9668-x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28508
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Title
Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures
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Abstract
We established cell suspension cultures derived from leaf, stem, and root calli of Pueraria candollei var. candollei and P. candollei var. mirifica using liquid Murashige and Skoog (MS) medium supplemented with 0.56 μM 6-benzyladenine (BA) and 4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Growth of the cell suspension cultures progressed to the stationary phase within 15-24 days. Methanolic extracts of cell suspension cultures of both varieties of P. candollei were analyzed using a validated HPLC protocol. All cell lines derived from leaf, stem, and root explants produced four major isoflavonoids: daidzein, daidzin, genistein, and genistin; these isoflavonoids were detected only in the roots of intact plants. Furthermore, the isoflavonoid contents of the cell suspension cultures were higher than those of intact plants. Thus, cell suspension culture of both varieties of P. candollei may be an effective tool for isoflavonoid production. © 2010 Springer Science+Business Media B.V.
