Publication: Assessment of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification and biotyping of the pathogenic oomycete Pythium insidiosum
2
Issued Date
2018-12-01
Resource Type
ISSN
18783511
12019712
12019712
Other identifier(s)
2-s2.0-85055732208
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Infectious Diseases. Vol.77, (2018), 61-67
Suggested Citation
Theerapong Krajaejun, Tassanee Lohnoo, Paisan Jittorntam, Atthapol Srimongkol, Yothin Kumsang, Wanta Yingyong, Thidarat Rujirawat, Onrapak Reamtong, Suthee Mangmee Assessment of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification and biotyping of the pathogenic oomycete Pythium insidiosum. International Journal of Infectious Diseases. Vol.77, (2018), 61-67. doi:10.1016/j.ijid.2018.09.006 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/46137
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Assessment of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification and biotyping of the pathogenic oomycete Pythium insidiosum
Other Contributor(s)
Abstract
© 2018 The Author(s) Objective: Pythiosis is a life-threatening infectious disease caused by the oomycete Pythium insidiosum. The disease has been increasingly reported worldwide. Most patients with pythiosis undergo surgical removal of an infected organ. Early diagnosis contributes to better prognosis of pythiosis patients. Here, we assessed the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification and biotyping of P. insidiosum. Methods: A comprehensive set of mass spectra of P. insidiosum was generated to evaluate MALDI-TOF MS for identification and biotyping of P. insidiosum. Results: MALDI-TOF MS accurately identified all 13 P. insidiosum strains tested, at the species level. Mass spectra of P. insidiosum did not match any other microorganisms, including fungi (i.e., Aspergillus species, Fusarium species, and fungal species of the class Zygomycetes), which have similar microscopic morphologies with this oomycete. MALDI-TOF MS- and rDNA sequence-based biotyping methods consistently classified P. insidiosum into three groups: Clade-I (American strains), II (Asian and Australian strains), and III (mostly Thai strains). Conclusions: MALDI-TOF MS has been successfully used for identification and biotyping of P. insidiosum. The obtained mass spectral database allows clinical microbiology laboratories, well-equipped with a MALDI-TOF mass spectrometer, to conveniently identify P. insidiosum, without requiring any pathogen-specific reagents (i.e., antigen, antibody or primers).