Publication: In vitro study of vancomycin release and osteoblast-like cell growth on structured calcium phosphate-collagen
Issued Date
2013-04-01
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ISSN
09284931
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2-s2.0-84873408093
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Mahidol University
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SCOPUS
Bibliographic Citation
Materials Science and Engineering C. Vol.33, No.3 (2013), 1423-1431
Suggested Citation
Weeraphat Pon-On, Narattaphol Charoenphandhu, Jarinthorn Teerapornpuntakit, Jirawan Thongbunchoo, Nateetip Krishnamra, I. Ming Tang In vitro study of vancomycin release and osteoblast-like cell growth on structured calcium phosphate-collagen. Materials Science and Engineering C. Vol.33, No.3 (2013), 1423-1431. doi:10.1016/j.msec.2012.12.046 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31759
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Title
In vitro study of vancomycin release and osteoblast-like cell growth on structured calcium phosphate-collagen
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Abstract
A drug delivery vehicle consisting of spherical calcium phosphate-collagen particles covered by flower-like (SFCaPCol) blossoms composed of nanorod building blocks and their cellular response is studied. The spherical structure was achieved by a combination of sonication and freeze-drying. The SFCaPCol blossoms have a high surface area of approximately 280 m2g- 1. The blossom-like formation having a high surface area allows a drug loading efficiency of 77.82%. The release profile for one drug, vancomycin (VCM), shows long term sustained release in simulated body fluid (SBF), in a phosphate buffer saline (PBS, pH 7.4) solution and in culture media over 2 weeks with a cumulative release ∼ 53%, 75% and 50%, respectively, over the first 7 days. The biocompatibility of the VCM-loaded SFCaPCol scaffold was determined by in vitro cell adhesion and proliferation tests of rat osteoblast-like UMR-106 cells. MTT tests indicated that UMR-106 cells were viable after exposure to the VCM loaded SFCaPCol, meaning that the scaffold (the flower-like blossoms) did not impair the cell's viability. The density of cells on the substrate was seen to increase with increasing cultured time. © 2012 Elsevier B.V. All Rights Reserved.