Publication: In vitro maturation and fertilization of swamp buffalo oocytes and their subsequent development
Issued Date
1992-01-01
Resource Type
ISSN
0093691X
Other identifier(s)
2-s2.0-38249010277
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Theriogenology. Vol.38, No.3 (1992), 545-555
Suggested Citation
K. Pavasuthipaisit, Y. Kitiyanant, C. Thonabulsombat, C. Tocharus, S. Sriurairatna, K. L. White In vitro maturation and fertilization of swamp buffalo oocytes and their subsequent development. Theriogenology. Vol.38, No.3 (1992), 545-555. doi:10.1016/0093-691X(92)90074-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22242
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Title
In vitro maturation and fertilization of swamp buffalo oocytes and their subsequent development
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Abstract
The present experiment was carried out to evaluate the maturation, fertilization and subsequent embryo culture of swamp buffalo oocytes in vitro. The oocytes (n=273) were collected and morphologically graded based on the structure of cumulus-oocyte complexes as Grade 1 (compact, n=81), Grade 2 (expanded, n=70), Grade 3 (partially denuded, n=65) or Grade 4 (completely denuded, n=57). More than 60% of the in vitro matured oocytes co-cultured with capacitated spermatozoa demonstrated evidence of fertilization or cleavage to the 2-cell stage when either Grade 1 or 2 oocytes were used. The percentage of fertilized oocytes undergoing 2-cell stage cleavages from Grade 3 (53%) and Grade 4 (46%) groups was significantly lower (P<0.01) than that observed in the Grade 1 (64%) and Grade 2 (68%) groups. Development to the 6 to 8 cell stage substantiated fertilization of Grade 1 and 2 oocytes. These results demonstrated that swamp buffalo oocytes are capable of maturing in vitro, forming embryos, and developing at least to the 8-cell stage in culture medium alone. © 1992.