Publication: Retroviral reverse transcriptase inhibitory activity in Thai herbs and spices: Screening with moloney murine leukemia viral enzyme
2
Issued Date
1993-12-01
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ISSN
00383619
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2-s2.0-0027860418
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.24, No.4 (1993), 751-755
Suggested Citation
O. Suthienkul, O. Miyazaki, M. Chulasiri, U. Kositanont, K. Oishi Retroviral reverse transcriptase inhibitory activity in Thai herbs and spices: Screening with moloney murine leukemia viral enzyme. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.24, No.4 (1993), 751-755. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/22620
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Title
Retroviral reverse transcriptase inhibitory activity in Thai herbs and spices: Screening with moloney murine leukemia viral enzyme
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Abstract
Fifty-seven Thai herbs and spices were examined for their retroviral reverse transcriptase inhibitory activity. All herbs and spices were extracted with hot-water and methanol. Reverse transcriptase inhibitory activity of the extracts was determined by using Moloney Murine Leukemia Virus reverse transcriptase (M-MuLV-RT) reacted with 3H-dTTP and radioactivity measured with a scintillation counter. Eighty-one per cent (46/57) of hot-water extracts and 54% (31/57) of methanol extracts showed inhibitory activities. At a concentration of 125 μg/ml, 13% (6/46) of hot-water extracts, namely Eugenia caryophyllus Bullock et Harrison, Phyllanthus urinaria Linn., Terminalia belerica Roxb., Nelumbo nucifera Gaertn., Psidium guajava Linn. and Lawsonia inermis Linn., had a relative inhibitory ratio (IR) over 50%. They showed ratios of 100%, 91%, 75%, 74%, 61% and 60%, respectively. For methanol extracts, only 10% (3/31) had IR values over 50%. They were T. belerica, E. caryoophyllus and N. nucifera which exhibited IR values of 83%, 54% and 54%, respectively.