Publication: The suitability of laboratory‑bred Anopheles cracens for the production of Plasmodium vivax sporozoites
Issued Date
2015
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eng
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Mahidol University
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BioMed Central
Bibliographic Citation
Malaria Journal. Vol.14, (2015), 312
Suggested Citation
Chiara Andolina, Jordi Landier, Verena Carrara, Chuม Cindy S, Franetich, Jean‑François, Alison Roth, Laurent Rénia, Clémentine Roucher, White, Nick J, Georges Snounou, François Nosten The suitability of laboratory‑bred Anopheles cracens for the production of Plasmodium vivax sporozoites. Malaria Journal. Vol.14, (2015), 312. doi:10.1186/s12936-015-0830-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/3096
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Title
The suitability of laboratory‑bred Anopheles cracens for the production of Plasmodium vivax sporozoites
Abstract
Background: A stenogamous colony of Anopheles cracens (A. dirus B) established 20 years ago in a Thai insectary
proved susceptible to Plasmodium vivax. However, routine sporozoite production by feeding on field-collected blood
samples has not been described. The setting-up of an A. cracens colony in an insectary on the Thai-Myanmar border
and the process of using P. vivax field samples for the production of infectious sporozoites are described.
Methods: The colony was started in 2012 from egg batches that were sent from the Department of Parasitology, Faculty
of Medicine, University of Chiang Mai, to the Shoklo Malaria Research Unit (SMRU), on wet filter paper in sealed
Petri dishes. From May 2013 to December 2014, P. vivax-infected blood samples collected from patients seeking care
at SMRU clinics were used for membrane feeding assays and sporozoite production.
Results: Mosquitoes were fed on blood samples from 55 patients, and for 38 (69 %) this led to the production
sporozoites. The average number of sporozoites obtained per mosquito was 26,112 (range 328–79,310). Gametocytaemia
was not correlated with mosquito infectiousness (p = 0.82), or with the number of the sporozoites produced
(Spearman’s ρ = −0.016, p = 0.905). Infectiousness did not vary with the date of collection or the age of the patient.
Mosquito survival was not correlated with sporozoite load (Spearman’s ρ = 0.179, p = 0.282).
Conclusion: Consistent and routine P. vivax sporozoites production confirms that A. cracens is highly susceptible to P.
vivax infection. Laboratory-bred colonies of this vector are suitable for experimental transmission protocols and thus
constitute a valuable resource.