Publication: Promising approach to reducing Malaria transmission by ivermectin: Sporontocidal effect against Plasmodium vivax in the South American vectors Anopheles aquasalis and Anopheles darlingi
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Issued Date
2018-02-14
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ISSN
19352735
19352727
19352727
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2-s2.0-85044386493
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Mahidol University
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SCOPUS
Bibliographic Citation
PLoS Neglected Tropical Diseases. Vol.12, No.2 (2018)
Suggested Citation
Yudi T. Pinilla, Stefanie C. P. Lopes, Vanderson S. Sampaio, Francys S. Andrade, Gisely C. Melo, Alessandra S. Orfanó, Nágila F.C. Secundino, Maria G.V.B. Guerra, Marcus V.G. Lacerda, Kevin C. Kobylinski, Karin S. Escobedo-Vargas, Victor M. López-Sifuentes, Craig A. Stoops, G. Christian Baldeviano, Joel Tarning, Gissella M. Vasquez, Paulo F.P. Pimenta, Wuelton M. Monteiro Promising approach to reducing Malaria transmission by ivermectin: Sporontocidal effect against Plasmodium vivax in the South American vectors Anopheles aquasalis and Anopheles darlingi. PLoS Neglected Tropical Diseases. Vol.12, No.2 (2018). doi:10.1371/journal.pntd.0006221 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/46944
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Title
Promising approach to reducing Malaria transmission by ivermectin: Sporontocidal effect against Plasmodium vivax in the South American vectors Anopheles aquasalis and Anopheles darlingi
Author(s)
Yudi T. Pinilla
Stefanie C. P. Lopes
Vanderson S. Sampaio
Francys S. Andrade
Gisely C. Melo
Alessandra S. Orfanó
Nágila F.C. Secundino
Maria G.V.B. Guerra
Marcus V.G. Lacerda
Kevin C. Kobylinski
Karin S. Escobedo-Vargas
Victor M. López-Sifuentes
Craig A. Stoops
G. Christian Baldeviano
Joel Tarning
Gissella M. Vasquez
Paulo F.P. Pimenta
Wuelton M. Monteiro
Stefanie C. P. Lopes
Vanderson S. Sampaio
Francys S. Andrade
Gisely C. Melo
Alessandra S. Orfanó
Nágila F.C. Secundino
Maria G.V.B. Guerra
Marcus V.G. Lacerda
Kevin C. Kobylinski
Karin S. Escobedo-Vargas
Victor M. López-Sifuentes
Craig A. Stoops
G. Christian Baldeviano
Joel Tarning
Gissella M. Vasquez
Paulo F.P. Pimenta
Wuelton M. Monteiro
Abstract
© 2018 Public Library of Science. All Rights Reserved. Background: The mosquito resistance to the insecticides threatens malaria control efforts, potentially becoming a major public health issue. Alternative methods like ivermectin (IVM) administration to humans has been suggested as a possible vector control to reduce Plasmodium transmission. Anopheles aquasalis and Anopheles darlingi are competent vectors for Plasmodium vivax, and they have been responsible for various malaria outbreaks in the coast of Brazil and the Amazon Region of South America. Methods: To determine the IVM susceptibility against P. vivax in An. aquasalis and An. darlingi, ivermectin were mixed in P. vivax infected blood: (1) Powdered IVM at four concentrations (0, 5, 10, 20 or 40 ng/mL). (2) Plasma (0 hours, 4 hours, 1 day, 5, 10 and 14 days) was collected from healthy volunteers after to administer a single oral dose of IVM (200 μg/kg) (3) Mosquitoes infected with P. vivax and after 4 days was provided with IVM plasma collected 4 hours post-treatment (4) P. vivax-infected patients were treated with various combinations of IVM, chloroquine, and primaquine and plasma or whole blood was collected at 4 hours. Seven days after the infective blood meal, mosquitoes were dissected to evaluate oocyst presence. Additionally, the ex vivo effects of IVM against asexual blood-stage P. vivax was evaluated. Results: IVM significantly reduced the prevalence of An. aquasalis that developed oocysts in 10 to 40 ng/mL pIVM concentrations and plasma 4 hours, 1 day and 5 days. In An. darlingi to 4 hours and 1 day. The An. aquasalis mortality was expressively increased in pIVM (40ng/mL) and plasma 4 hours, 1, 5 10 and 14 days post-intake drug and in An. darlingi only to 4 hours and 1 day. The double fed meal with mIVM by the mosquitoes has a considerable impact on the proportion of infected mosquitoes for 7 days post-feeding. The oocyst infection prevalence and intensity were notably reduced when mosquitoes ingested blood from P. vivax patients that ingested IVM+CQ, PQ+CQ and IVM+PQ+CQ. P. vivax asexual development was considerably inhibited by mIVM at four-fold dilutions. Conclusion: In conclusion, whole blood spiked with IVM reduced the infection rate of P. vivax in An. aquasalis and An. darlingi, and increased the mortality of mosquitoes. Plasma from healthy volunteers after IVM administration affect asexual P. vivax development. These findings support that ivermectin may be used to decrease P. vivax transmission.