Publication: A novel method and simple apparatus for the detection of thermophilic Campylobacter spp. in chicken meat products
Issued Date
2009-02-01
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ISSN
01677012
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2-s2.0-58349111681
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Microbiological Methods. Vol.76, No.2 (2009), 169-173
Suggested Citation
Sueptrakool Wisessombat, Kanokwan Kittiniyom, Potjanee Srimanote, Wijit Wonglumsom, Supayang P. Voravuthikunchai A novel method and simple apparatus for the detection of thermophilic Campylobacter spp. in chicken meat products. Journal of Microbiological Methods. Vol.76, No.2 (2009), 169-173. doi:10.1016/j.mimet.2008.10.007 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/27292
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Title
A novel method and simple apparatus for the detection of thermophilic Campylobacter spp. in chicken meat products
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Abstract
Conventional procedures for isolation of thermophilic Campylobacter spp. from chicken are complex, labor intensive, and time-consuming. The objective of this study was to create a novel Campylobacter culturing apparatus. A main concept of the device was based on the ability of Campylobacter to pass through a 0.45 μm pore size filter in viscous media. Preliminary study demonstrated that only viable Campylobacter moved through the membrane filter and could multiply in the enrichment culture. C. jejuni, C. coli, C. lari, and C. upsaliensis in the chicken samples were detected at cell concentrations as low as 10 cfu/g, after 24 h incubation at 42 °C. In total, 84 retail chicken samples were comparatively studied using both conventional method and apparatus. Sixteen samples (19.05%) were positive by the apparatus method; 14 (16.66%) of these positive samples contained C. coli and 2 (2.38%) contained C. jejuni. With the conventional method, 7 (8.33%) samples were positive 7 (8.33%) with C. coli. In conclusion, the apparatus detected more positive samples than did the conventional culture method. © 2008 Elsevier B.V. All rights reserved.