Publication: Effects of the Ayurved Siriraj Wattana recipe on functional and phenotypic characterization of cytokine-induced killer cells and dendritic cells in vitro
Issued Date
2016-11-29
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14726882
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2-s2.0-84999188134
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Mahidol University
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SCOPUS
Bibliographic Citation
BMC Complementary and Alternative Medicine. Vol.16, No.1 (2016)
Suggested Citation
Adisak Wongkajornsilp, Nuntarak Numchaisermsuk, Khanit Sa-ngiamsuntorn, Pravit Akarasereenont, Valla Wamanuttajinda, Kanda Kasetsinsombat, Sunisa Duangsa-ard, Tawee Laohapan, Kittipong Maneechotesuwan Effects of the Ayurved Siriraj Wattana recipe on functional and phenotypic characterization of cytokine-induced killer cells and dendritic cells in vitro. BMC Complementary and Alternative Medicine. Vol.16, No.1 (2016). doi:10.1186/s12906-016-1480-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/40975
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Title
Effects of the Ayurved Siriraj Wattana recipe on functional and phenotypic characterization of cytokine-induced killer cells and dendritic cells in vitro
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Abstract
© 2016 The Author(s). Background: Ayurved Siriraj Wattana recipe (AVS073), has been prescribed as tonic, to increase appetite, and for pain relief. It also exhibits antioxidant, anti-inflammatory, immunomodulating and anti-cancer activities. However, the immunomodulatory effects on antigen-presenting cells and effector T cells remained elusive. We thus aimed to study the effects of AVS073 on differentiation, maturation, functions and proportions of CIK cells and monocyte-derived DCs. Methods: CIK cells and monocyte-derived DCs were treated with AVS073, followed by the assessment of T-helper (Th) phenotypes using real-time RT-PCR and flow cytometry. Results: AVS073 promoted Th1 phenotype in CD3+CD56+ subset of CIK cells through increasing STAT4, T-bet, and interferon-γ. AVS073 inhibited Th2 phenotype through decreasing STAT6. AVS073 inhibited Treg phenotype through decreasing STAT5A, STAT5B and IDO. AVS073 promoted Th17 phenotype through increasing STAT3, RORC and IL-17. AVS073 treatment of mDCs resulted in increasing Th1-prone cytokine (IL-12) and Th17-prone cytokines (IL-6 and IL-23). Conclusions: AVS073 upregulated Th1 and Th17, but downregulated Th2 and Treg phenotypes within CD3+CD56+ cells. The treatment of mDCs drove Th1 and Th17-polarizations.