Publication: Chemical standardization and anti-proliferative activity of ardisia elliptica fruit against the HCT116 human colon cancer cell line
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Issued Date
2020-02-25
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ISSN
14203049
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2-s2.0-85081206064
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecules. Vol.25, No.5 (2020)
Suggested Citation
Suchanuch Ondee, Pongtip Sithisarn, Supachoke Mangmool, Piyanuch Rojsanga Chemical standardization and anti-proliferative activity of ardisia elliptica fruit against the HCT116 human colon cancer cell line. Molecules. Vol.25, No.5 (2020). doi:10.3390/molecules25051023 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/53583
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Title
Chemical standardization and anti-proliferative activity of ardisia elliptica fruit against the HCT116 human colon cancer cell line
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Abstract
© 2020 by the authors. The present study is intended to carry out the chemical standardization and evaluation of the anti-proliferative activity of A. elliptica fruit extract. A. elliptica fruit powder was extracted with ethanol. The obtained extract was assessed for total phenolic content using the Folin–Ciocalteu method. Moreover, a simple, accurate, and precise reversed phase high-performance liquid chromatographic method was developed and validated to determine the embelin content of A. elliptica fruit extract. Then, the extract and embelin were investigated for their anti-proliferative effect against HCT-116 cells. Finally, the mechanisms of inhibition of the extract and embelin on the mRNA expression of pro-apoptotic genes Bad, Bax, and Caspase-8 and anti-apoptotic genes c-IAP1, Mcl-1, and XIAP were determined by real-time qRT-PCR. The phenolic content and embelin content of the extract were 5.20 ± 0.01 g of gallic acid equivalent per 100 g of dried fruit (g% GAE) and 5.57 ± 0.56 mg/g of extract, respectively. The extract and embelin showed strong anti-proliferative effects on HCT-116 cells with 50% inhibition concentration (IC50) values of 19.16 ± 1.09 µg/mL and 25.93 ± 1.75 µg/mL, respectively. The A. elliptica extract exhibited a significant increase in the mRNA level of Bad, Bax, and Caspase-8 and a significant decrease in c-IAP1, Mcl-1, and XIAP. Embelin showed a significant decrease in Mcl-1 and XIAP.