Publication: Simultaneous HPTLC determination of rhein and aloe-emodin in senna alata leaves from Thailand and their commercial products
Issued Date
2017-01-01
Resource Type
ISSN
15559475
1934578X
1934578X
Other identifier(s)
2-s2.0-85019104902
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Natural Product Communications. Vol.12, No.3 (2017), 399-401
Suggested Citation
Savita Chewchinda, Pongtip Sithisarn Simultaneous HPTLC determination of rhein and aloe-emodin in senna alata leaves from Thailand and their commercial products. Natural Product Communications. Vol.12, No.3 (2017), 399-401. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/41574
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Simultaneous HPTLC determination of rhein and aloe-emodin in senna alata leaves from Thailand and their commercial products
Author(s)
Other Contributor(s)
Abstract
A high performance thin layer chromatographic (HPTLC) method was developed and validated for simultaneous determination of rhein and aloe-emodin, major anthraquinone constituents, in S. alata leaves. The separation was performed on a silica gel 60 F254 HPTLC plate using ethyl acetate: methanol: water (100:17:10, v/v/v) as the development system. Validation of the analytical method for rhein and aloe-emodin promoted acceptable parameters. Good linearity in the range of 40-480 ng/band was obtained while intra-day and inter-day precisions were shown to be precise with relative standard deviations of less than 5%. The average percentage recoveries of rhein and aloe-emodin were 98.8% and 98.9%, suggesting acceptable accuracy. The content of rhein and aloe-emodin in S. alata leaves collected from 5 different provinces in Thailand analyzed by the validated HPTLC method were in the ranges of 0.098 ± 0.017 - 0.30 ± 0.02%, w/w, and 0.081 ± 0.0006 - 0.34 ± 0.0009 %, w/w, respectively. Five commercial products of S. alata tea available in the market were purchased and analyzed for rhein and aloe-emodin contents. The contents of rhein and aloe-emodin in the tea samples were in the ranges of 0.085 ± 0.004 - 0.23 ± 0.04%, w/w, and 0.096 ± 0.006 - 0.30 ± 0.01 %, w/w, respectively. The HPTLC method is rapid, reliable, sensitive and economical for routine analysis of rhein and aloe-emodin contents in S. alata leaf raw materials and its commercial products.