Publication: Specific immunomodulatory and secretory activities of stevioside and steviol in intestinal cells
Issued Date
2008-05-28
Resource Type
ISSN
00218561
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2-s2.0-45549104311
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Agricultural and Food Chemistry. Vol.56, No.10 (2008), 3777-3784
Suggested Citation
Chaiwat Boonkaewwan, Mei Ao, Chaivat Toskulkao, Mrinalini C. Rao Specific immunomodulatory and secretory activities of stevioside and steviol in intestinal cells. Journal of Agricultural and Food Chemistry. Vol.56, No.10 (2008), 3777-3784. doi:10.1021/jf072681o Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18737
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Title
Specific immunomodulatory and secretory activities of stevioside and steviol in intestinal cells
Abstract
Stevioside, isolated from Stevia rebaudiana, is a commercial sweetener. It was previously demonstrated that stevioside attenuates NF-κB-dependent TNF-α and IL-1β synthesis in LPS-stimulated monocytes. The present study examined the effects of stevioside and its metabolite, steviol, on human colon carcinoma cell lines. High concentrations of stevioside (2-5 mM) and steviol (0.2-0.8 mM) decreased cell viability in T84, Caco-2, and HT29 cells. Stevioside (2 mM) potentiated TNF-α-mediated IL-8 release in T84 cells. However, steviol (0.01-0.2 mM) significantly suppressed TNF-α-induced IL-8 release in all three cell lines. In T84 cells, steviol attenuated TNF-α-stimulated IκB → NF-κB signaling. Chloride transport was stimulated by steviol (0.1 mM) > stevioside (1 mM) at 30 min. Two biological effects of steviol in the colon are demonstrated for the first time: stimulation of Cl- secretion and attenuation of TNF-α-stimulated IL-8 production. The immunomodulatory effects of steviol appear to involve NF-κB signaling. In contrast, at nontoxic concentrations stevioside affects only Cl- secretion. © 2008 American Chemical Society.