Publication: Analyses of vaccination protocols for Leptospira interrogans serovar Autumnalis in hamsters
Issued Date
2008-11-01
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ISSN
00029637
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2-s2.0-55849098593
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.79, No.5 (2008), 779-786
Suggested Citation
Amporn Srikram, Surasakdi Wongratanacheewin, Anucha Puapairoj, Vanaporn Wuthiekanun, Rasana W. Sermswan Analyses of vaccination protocols for Leptospira interrogans serovar Autumnalis in hamsters. American Journal of Tropical Medicine and Hygiene. Vol.79, No.5 (2008), 779-786. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/19280
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Title
Analyses of vaccination protocols for Leptospira interrogans serovar Autumnalis in hamsters
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Abstract
Leptospirosis, caused by Leptospira spp., is a zoonotic disease found worldwide. Killed whole cell leptospiral vaccines have been used as effective vaccines to elicit specific antibodies for protection. However, the involvement of cytokine responses after vaccination is not well characterized. Hamsters were immunized with killed L. interrogans serovar Autumnalis before challenge to study cytokine mRNA expression levels (interferon [IFN]-γ, tumor necrosis factor [TNF]-α, interleukin [IL]-10, and IL-4). Vaccinated groups showed 92-100% survival rates, whereas control hamsters died within 6-10 days. However, live organisms were detected in vaccinated groups, and mild to moderate pathology was observed early in infection. IFN-γ and TNF-α mRNA expression levels correlated with the severity of infection and lung pathology, whereas IL-4 and IL-10 expression levels were significantly higher in vaccinated groups. In summary, commonly used vaccines changed the cytokine profiles and protected hamsters from death but failed to stimulate sterile immunity and were unable to prevent the occurrence of pathology. Copyright © 2008 by The American Society of Tropical Medicine and Hygiene.