Publication: Kidney stone inhibitors in patients with renal stones and endemic renal tabular acidosis in northeast Thailand
Issued Date
2004-05-01
Resource Type
ISSN
03005623
Other identifier(s)
2-s2.0-3142679405
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Mahidol University
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SCOPUS
Bibliographic Citation
Urological Research. Vol.32, No.2 (2004), 112-116
Suggested Citation
Yasushi Nakagawa, Mauricio Carvalho, Prida Malasit, Sumalee Nimmannit, Suchai Sritippaywan, Somkiat Vasuvattakul, Somchai Chutipongtanate, Vipada Chaowagul, Sanga Nilwarangkur Kidney stone inhibitors in patients with renal stones and endemic renal tabular acidosis in northeast Thailand. Urological Research. Vol.32, No.2 (2004), 112-116. doi:10.1007/s00240-003-0389-z Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21670
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Title
Kidney stone inhibitors in patients with renal stones and endemic renal tabular acidosis in northeast Thailand
Abstract
Distal renal tubular acidosis (dRTA) is generally associated with hypercalciuria, hypocitraturia, and nephrolithiasis. Our intention was to study glycosaminoglycans (GAGS) and nephrocalcin (NC), two well-known crystal growth inhibitors, in a population with endemic dRTA and nephrolithiasis in northeast (NE) Thailand. We studied 13 patients, six with dRTA and seven with nephrolithiasis with normal or undefined acidification function. Six healthy adults living in the same area as the patients and another six from the Bangkok (BKK) area were used as controls. We measured urinary pH, ammonia, calcium, citrate, magnesium, oxalate, potassium, sodium and uric acid. GAGS were determined by an Alcian blue precipitation method and were qualitated by agarose gel electrophoresis after being isolated using 5% cetyltrimethylammonium bromide at pH 6.0. NC isoforms were isolated as previously described by Nakagawa et al. Citrate was higher in BKK controls (p < 0.04). There was a striking difference among GAGS from BKK when compared with other groups (103.85 ± 10.70 vs. 23.52 ± 8.11 for dRTA, 22.36 ± 14.98 for kidney stone patients and 14.73 ± 2.87 mg/ml in controls from the NE region, (p < 0.0001). dRTA and stone-forming patients excrete proportionally more (C + D) than (A + B) NC isoforms (p < 0.05). Also, their NC showed a 100-fold weaker binding capacity of calcium oxalate monohydrate crystals. The ratio of chondroitin sulfate/heparin sulfate in GAGS was approximately 9/1. In addition to the traditional risk factors for nephrolithiasis in dRTA, GAGS and NC might play an important role in the pathogenesis of stone formation in this population.