Publication:
Effect of storage levels of nitric oxide derivatives in blood components

dc.contributor.authorAlan N. Schechteren_US
dc.contributor.authorMelissa A. Qazien_US
dc.contributor.authorFabiola Rizzattien_US
dc.contributor.authorBarbora Piknovaen_US
dc.contributor.authorNathawut Sibmoohen_US
dc.contributor.authorDavid F. Stronceken_US
dc.contributor.otherNational Institute of Diabetes and Digestive and Kidney Diseasesen_US
dc.contributor.otherUniversidade Federal de Sao Carlosen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherNational Institutes of Health, Bethesdaen_US
dc.date.accessioned2018-06-11T04:33:12Z
dc.date.available2018-06-11T04:33:12Z
dc.date.issued2012-10-22en_US
dc.description.abstractBackground: Potential deleterious effects of red blood cell (RBC) transfusions, especially from blood kept at length, have been ascribed to biochemical changes during storage, including those of nitric oxide (NO) metabolism. Study methods and design: In this study, NO metabolites, nitrite and nitrate, were quantified in RBCs and whole blood with time of storage. Whole blood (WB), leukoreduced (LR), and non-leukoreduced (NLR) components were obtained from healthy volunteer donors and stored in polyvinyl chloride bags for 42 days. Nitrite and nitrate were measured using reductive gas-phase chemiluminescence. Results: Nitrite concentrations initially decreased rapidly from about 150nmol/L, but stabilized at about 44nmol/L in room air for up to 42 days. Nitrate concentrations remained stable during storage at about 35μmol/L. Cells from bags maintained in an argon chamber showed decreased nitrite levels compared to those maintained in room air. Inhibition of enzymes implicated in the NO cycle did not alter nitrite levels. Conclusion: As erythrocytes may contribute to the control of blood flow and oxygen delivery through reduction of nitrite to NO under hypoxic conditions, the present findings provide insight into possible effects of blood transfusion. These measurements may explain some adverse effects of RBC transfusion and suggest ways of optimizing the preservation of stored blood. © 2012 Qazi MA et al.en_US
dc.identifier.citationF1000Research. Vol.1, (2012)en_US
dc.identifier.doi10.12688/f1000research.1-35.v1en_US
dc.identifier.issn20461402en_US
dc.identifier.other2-s2.0-84897641860en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/13589
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84897641860&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectPharmacology, Toxicology and Pharmaceuticsen_US
dc.titleEffect of storage levels of nitric oxide derivatives in blood componentsen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84897641860&origin=inwarden_US

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