Publication:
Generalized and mobilizable positive-selection cloning vectors

Issued Date

1994-05-27

Resource Type

Article

ISSN

03781119

DOI

10.1016/0378-1119(94)90620-3

Other identifier(s)

2-s2.0-0028260594

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Gene. Vol.143, No.1 (1994), 145-146

Suggested Citation

Skorn Mongkolsuk, Siritida Rabibhadana, Paiboon Vattanaviboon, Suvit Loprasert Generalized and mobilizable positive-selection cloning vectors. Gene. Vol.143, No.1 (1994), 145-146. doi:10.1016/0378-1119(94)90620-3 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/9511

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Title

Generalized and mobilizable positive-selection cloning vectors

Author(s)

Skorn Mongkolsuk
 Siritida Rabibhadana
 Paiboon Vattanaviboon
 Suvit Loprasert

Other Contributor(s)

Chulabhorn Research Institute
 Mahidol University

Abstract

We have modified the positive-selection cloning vector pUN121 to expand the numbers of unique cloning sites by insertion of a multiple cloning site into the λ, ficI gene without disrupting its repressor function, resulting in plasmid pSKM10. Plasmid pSKM10 has seven restriction enzyme sites suitable for general cloning purposes. A mobilizable version (pSKM11) of pSKM10 was constructed by insertion of the IncP mob sequence which permitted mobilization of the plasmid into a wide variety of Gram - bacteria. © 1994.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/9511

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Scopus 1991-2000