Publication: Assessment of Plasmodium antigens and CRP in dried blood spots with multiplex malaria array
Issued Date
2021-06-01
Resource Type
ISSN
09750703
09717196
09717196
Other identifier(s)
2-s2.0-85098580125
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Parasitic Diseases. Vol.45, No.2 (2021), 479-489
Suggested Citation
Ihn Kyung Jang, Sara Aranda, Rebecca Barney, Andrew Rashid, Muhammad Helwany, John C. Rek, Emmanuel Arinaitwe, Harriet Adrama, Maxwell Murphy, Mallika Imwong, Stephane Proux, Warat Haohankhunnatham, Xavier C. Ding, François Nosten, Bryan Greenhouse, Dionicia Gamboa, Gonzalo J. Domingo Assessment of Plasmodium antigens and CRP in dried blood spots with multiplex malaria array. Journal of Parasitic Diseases. Vol.45, No.2 (2021), 479-489. doi:10.1007/s12639-020-01325-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/77283
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Title
Assessment of Plasmodium antigens and CRP in dried blood spots with multiplex malaria array
Other Contributor(s)
Faculty of Tropical Medicine, Mahidol University
Infectious Diseases Research Collaboration
Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander von Humboldt
Foundation for Innovative New Diagnostics, Switzerland
PATH Seattle
University of California, San Francisco
Nuffield Department of Medicine
Infectious Diseases Research Collaboration
Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander von Humboldt
Foundation for Innovative New Diagnostics, Switzerland
PATH Seattle
University of California, San Francisco
Nuffield Department of Medicine
Abstract
Dried blood spots (DBS) typically prepared on filter papers are an ideal sample type for malaria surveillance by offering easy and cost-effective methods in terms of sample collection, storage, and transport. The objective of this study was to evaluate the applicability of DBS with a commercial multiplex malaria assay, developed to concurrently measure Plasmodium antigens, histidine-rich protein 2 (HRP2), Plasmodium lactate dehydrogenase (pLDH), and a host inflammatory biomarker, C-reactive protein (CRP), in whole blood. The assay conditions were optimized for DBS, and thermal stability for measurement of Plasmodium antigens and CRP in dried blood were determined. Performance of the multiplex assay on matched DBS and whole blood pellet samples was also evaluated using the clinical samples. The results indicate the acceptable performance in multiplex antigen detection using DBS samples. At cutoff levels for DBS, with a diagnostic specificity with a lower 95% confidence bound > 92%, diagnostic sensitivities against polymerase chain reaction (PCR)–confirmed malaria for HRP2, Pf LDH, Pv LDH, and Pan LDH were 93.5%, 80.4%, 21.3%, and 55.6%, respectively. The half-life of pLDH was significantly less than that of HRP2 in thermal stability studies. Results with DBS samples collected from Peru indicate that the uncontrolled storage conditions of DBS can result in inaccurate reporting for infection with P. falciparum parasites with hrp2/3 deletions. With careful consideration that minimizing the unfavorable DBS storage environment is essential for ensuring integrity of heat-labile Plasmodium antigens, DBS samples can be used as an alternative to liquid whole blood to detect P. falciparum with hrp2/3 deletions in malaria surveillance.