Publication: Assessment of appropriate l-cysteine concentration for boar semen cryopreservation by using flow cytometry
Issued Date
2012-12-01
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ISSN
01256491
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2-s2.0-84884575628
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Mahidol University
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SCOPUS
Bibliographic Citation
Thai Journal of Veterinary Medicine. Vol.42, No.4 (2012), 483-488
Suggested Citation
Paisan Tienthai, Panida Chanapiwat, Sukanya Manee-in, Dulyatad Gronsang, Kampon Kaeoket Assessment of appropriate l-cysteine concentration for boar semen cryopreservation by using flow cytometry. Thai Journal of Veterinary Medicine. Vol.42, No.4 (2012), 483-488. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15224
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Title
Assessment of appropriate l-cysteine concentration for boar semen cryopreservation by using flow cytometry
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Abstract
The present study aims to investigate a suitable L-cysteine level to be added the lactose-egg yolk (LEY) extender to improve the quality of boar semen after freezing-thawing using Annexin-V and propidium iodide (PI) assay detected with flow cytometer. Ejaculates from twelve boars were processed in the LEY extender and added with 0, 5, 10 and 15 mM of L-cysteine. Semen suspensions were cryopreserved with a controlled-rate freezer and thawed for examination. The surface of sperm plasma membrane was explored by scanning electron microscopy (SEM) while sperm membrane integrity was performed by Annexin-V/PI assay. Of all treatments, the damaged spermatozoa in various patterns were observed whereas the intact spermatozoa were still found by SEM. The viable and non-apoptotic (AN-/PI-) sperms were higher (p < 0.05) in the levels of 5 and 10 mM. These results indicated that the damaged spermatozoa were found after freezing/thawing and the L-cysteine at concentrations of 5 to 10 mM added to LEY extender was the optimal dose against oxidative stress and acquisition for improving cryopreserved boar sperm quality.