Publication: Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis
Issued Date
1990-06-18
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ISSN
00951137
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2-s2.0-0025287841
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Clinical Microbiology. Vol.28, No.6 (1990), 1249-1253
Suggested Citation
M. Kunakorn, P. Boonma, K. Khupulsup, B. Petchclai Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis. Journal of Clinical Microbiology. Vol.28, No.6 (1990), 1249-1253. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15957
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Title
Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis
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Abstract
Indirect hemagglutination (IHA) is commonly used for serodiagnosis of melioidosis. However, in endemic areas, high background titers in normal populations and occasional low titers in patients with septicemic melioidosis prompted a search for a more sensitive and more specific method of serodiagnosis. An indirect fluorescent-antibody test for immunoglobulin M (IgM) specific antibody to Pseudomonas pseudomallei was more sensitive and more specific, but fluorescence microscopes are rarely available in the endemic areas. An enzyme-linked immunosorbent assay (ELISA) for IgM antibody is an attractive alternative. An indirect ELISA for IgM antibody (IgM ELISA) and an IgM antibody capture ELISA for melioidosis were developed. Both tests, together with IHA, were evaluated for 153 serum specimens from blood donors and 16 serum specimens from 16 melioidosis patients. It was found that IHA, the IgM ELISA, and the IgM antibody capture ELISA had sensitivities of 88, 88, and 75%, respectively, with specificities of 97.4, 92.2, and 91.5%, respectively. When IHA was combined with IgM ELISA, a sensitivity of 100% and a specificity of 95.4% were obtained. The IgM ELISA and IHA should be used in combination for serodiagnosis of melioidosis.