Publication:
High-resolution melting curve analysis for genotyping of common SNP in MTHFR gene using fixed-cell suspension

dc.contributor.authorThivaratana Sinthuwiwaten_US
dc.contributor.authorPhanasit Poowasanpetchen_US
dc.contributor.authorAngsana Wongngamrungrojen_US
dc.contributor.authorSomying Promsoen_US
dc.contributor.authorChirayu Auewarakulen_US
dc.contributor.authorSean Mooneyen_US
dc.contributor.authorChintana Tocharoentanapholen_US
dc.contributor.otherChulabhorn Research Instituteen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherFaculty of Medicine, Siriraj Hospital, Mahidol Universityen_US
dc.contributor.otherIndiana University School of Medicine Indianapolisen_US
dc.date.accessioned2018-07-12T02:16:59Z
dc.date.available2018-07-12T02:16:59Z
dc.date.issued2008-10-01en_US
dc.description.abstractGenetic variation in MTHFR might explain the interindividual differences in both therapeutic and toxic responses to the treatment of cancer and rheumatoid arthritis with methotrexate, and can be involved in the sensitivity of developing diseases like cancer and congenital anomalies. We investigated the common sequence variation, C677T, in the MTHFR gene in fixed-cell specimens archived after chromosomal analysis using a novel gene scanning method based on post PCR analysis of high-resolution melting curves (HRM). These fixed specimens were stored after routine chromosomal analysis for 1 year at -20 °C in a 3:1 methanol:acetic acid solution. The method revealed a distinct pattern between homozygous and heterozygous alleles. Sensitivity and specificity of the HRM based method were comparable to that obtained by a hybridization probe. While the success rate for genotyping of a common SNP in MTHFR was similar to the hybridization probe approach, the HRM based method was more cost-effective and had a shorter turnaround time. © 2008 Elsevier Ltd.en_US
dc.identifier.citationMolecular and Cellular Probes. Vol.22, No.5-6 (2008), 329-332en_US
dc.identifier.doi10.1016/j.mcp.2008.07.004en_US
dc.identifier.issn08908508en_US
dc.identifier.other2-s2.0-56749164913en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/18848
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=56749164913&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleHigh-resolution melting curve analysis for genotyping of common SNP in MTHFR gene using fixed-cell suspensionen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=56749164913&origin=inwarden_US
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