Publication: Comparative proteomic analysis of human cholangiocarcinoma cell lines: S100A2 as a potential candidate protein inducer of invasion
Issued Date
2015-01-01
Resource Type
ISSN
18758630
02780240
02780240
Other identifier(s)
2-s2.0-84929347273
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Disease Markers. Vol.2015, (2015)
Suggested Citation
Kasima Wasuworawong, Sittiruk Roytrakul, Atchara Paemanee, Kattaleeya Jindapornprasert, Waraporn Komyod Comparative proteomic analysis of human cholangiocarcinoma cell lines: S100A2 as a potential candidate protein inducer of invasion. Disease Markers. Vol.2015, (2015). doi:10.1155/2015/629367 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35541
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Comparative proteomic analysis of human cholangiocarcinoma cell lines: S100A2 as a potential candidate protein inducer of invasion
Other Contributor(s)
Abstract
© 2015 Kasima Wasuworawong et al. Cholangiocarcinoma (CCA) is a bile duct cancer, commonly found in Asia including Thailand and especially in the northeastern region of Thailand. To identify the proteins involved in carcinogenesis and metastasis of CCA, protein expression profiles of high-invasive KKU-M213 and low-invasive KKU-100 cell lines were compared using a comparative Ge LC-MS/MS proteomics analysis. A total of 651 differentially expressed proteins were detected of which 27 protein candidates were identified as having functions involved in cell motility. A total of 22 proteins were significantly upregulated in KKU-M213, whereas 5 proteins were downregulated in KKU-M213. S100A2, a calcium-binding protein in S100 protein family, is upregulated in KKU-M213. S100A2 is implicated in metastasis development in several cancers. The protein expression level of S100A2 was verified by Western blot analysis. Intriguingly, high-invasive KKU-M213 cells showed higher expression of S100A2 than KKU-100 cells, consistent with proteomic data, suggesting that S100A2may be a key protein involved in the progression of CCA. However, the biological function of S100A2 in cholangiocarcinoma remains to be elucidated. S100A2 might be a potential biomarker as well as a novel therapeutic target in CCA metastasis.