Publication: Genome-Wide Association Study of HIV-Related Lipoatrophy in Thai Patients: Association of a DLGAP1 Polymorphism with Fat Loss
Issued Date
2015-08-01
Resource Type
ISSN
19318405
08892229
08892229
Other identifier(s)
2-s2.0-84938839112
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
AIDS Research and Human Retroviruses. Vol.31, No.8 (2015), 792-796
Suggested Citation
Sumonmal Uttayamakul, Tiphaine Oudot-Mellakh, Emi E. Nakayama, Pimrapat Tengtrakulcharoen, Julien Guergnon, Jean Francois Delfraissy, Srisin Khusmith, Chariya Sangsajja, Sirirat Likanonsakul, Ioannis Theodorou, Tatsuo Shioda Genome-Wide Association Study of HIV-Related Lipoatrophy in Thai Patients: Association of a DLGAP1 Polymorphism with Fat Loss. AIDS Research and Human Retroviruses. Vol.31, No.8 (2015), 792-796. doi:10.1089/aid.2014.0266 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/36092
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Genome-Wide Association Study of HIV-Related Lipoatrophy in Thai Patients: Association of a DLGAP1 Polymorphism with Fat Loss
Abstract
© Copyright 2015, Mary Ann Liebert, Inc. 2015. HIV-related lipoatrophy (LA) is a major adverse drug effect among HIV patients receiving the antiretroviral drug stavudine (d4T) in Southeast Asia. Although the development of LA could be observed in almost all HIV patients administered d4T for extended periods, there is considerable variation in the duration required to develop LA within this patient population. This study aimed to identify host genetic polymorphisms affecting the rate of LA onset in Thai HIV patients. We performed a genome-wide association study of HIV-related LA among patients at the Bamrasnaradura Infectious Diseases Institute, Thailand. Genotypes of HIV patients who developed LA within 2 years of treatment were compared with those of patients who did not develop LA after at least 4 years of treatment (non-LA patients). Genotypes of 49 LA and 92 non-LA patients at 578,525 single nucleotide polymorphisms (SNPs) were determined by Illumina bead arrays. The TaqMan real-time PCR method was used in a replication study. Five SNPs in the bead arrays, which showed the lowest p values in a comparison of LA with non-LA patients, were further tested in independent and sex-matched subpopulations consisting of 95 LA and 95 non-LA patients. This replication study revealed a significant association of LA with an SNP (rs12964965) in the gene encoding the Disks Large Homolog-Associated Protein 1 (DLGAP1), even after the correction for five multiple comparisons. These results strongly suggested involvement of the DLGAP1 gene product in the development of LA in Thai HIV patients.