Publication:
Acid‐extracted nuclear proteins and ultrastructure of human sperm chromatin as revealed by differential extraction with urea, mercaptoethanol, and salt

Issued Date

1981-01-01

Resource Type

Article

ISSN

15543919
01487280

DOI

10.1002/mrd.1120040405

Other identifier(s)

2-s2.0-0019725767

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Gamete Research. Vol.4, No.4 (1981), 297-315

Suggested Citation

Nongnuj Tanphaichitr, Prasert Sobhon, Panpaka Chalermisarachai, Malinee Patilantakarnkool Acid‐extracted nuclear proteins and ultrastructure of human sperm chromatin as revealed by differential extraction with urea, mercaptoethanol, and salt. Gamete Research. Vol.4, No.4 (1981), 297-315. doi:10.1002/mrd.1120040405 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/30148

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Title

Acid‐extracted nuclear proteins and ultrastructure of human sperm chromatin as revealed by differential extraction with urea, mercaptoethanol, and salt

Author(s)

Nongnuj Tanphaichitr
 Prasert Sobhon
 Panpaka Chalermisarachai
 Malinee Patilantakarnkool

Other Contributor(s)

Mahidol University

Abstract

Basic proteins were differentially extracted from the purified heads of ejaculated human sperm by successive treatment with (1) 1% Triton X‐100, 1% mercaptoethanol (ME); (2) 8 M urea, 1% ME; (3) 8 M urea, 1% ME, 0.2 M NaCl; and (4) 8 M urea, 1% ME, 0.6 M NaCl. Nonhistones were extracted in the first, second, and third treatments; histones, in the second and third; and most of the protamines, in the fourth, together with DNA. Corresponding electro microscopic studies of the sperm pellets after each treatment suggest that the nucleoprotamines are discrete portions of chromatin which are organized in the form of long thick cords and oval bodies of about 400–550 Å in diameter interlinked with very thin strands of fibers about 20–50 Å thick, which could represent the naked DNA depleted of its constituent histones. Copyright © 1981 Alan R. Liss, Inc.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

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URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/30148

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Scopus 1969-1990