Publication: Epigallocatechin-3-gallate protects pro-acinar epithelia against salivary gland radiation injury
Issued Date
2021-03-02
Resource Type
ISSN
14220067
16616596
16616596
Other identifier(s)
2-s2.0-85102696212
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Molecular Sciences. Vol.22, No.6 (2021), 1-15
Suggested Citation
Erni Sulistiyani, James M. Brimson, Ajjima Chansaenroj, Ladawan Sariya, Ganokon Urkasemsin, Sornjarod Oonsiri, Tewin Tencomnao, Anjalee Vacharaksa, Risa Chaisuparat, Joao N. Ferreira Epigallocatechin-3-gallate protects pro-acinar epithelia against salivary gland radiation injury. International Journal of Molecular Sciences. Vol.22, No.6 (2021), 1-15. doi:10.3390/ijms22063162 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76249
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Title
Epigallocatechin-3-gallate protects pro-acinar epithelia against salivary gland radiation injury
Abstract
Antioxidant agents are promising pharmaceuticals to prevent salivary gland (SG) epithelial injury from radiotherapy and their associated irreversible dry mouth symptoms. Epigallocatechin-3-gallate (EGCG) is a well-known antioxidant that can exert growth or inhibitory biological effects in normal or pathological tissues leading to disease prevention. The effects of EGCG in the various SG epithelial compartments are poorly understood during homeostasis and upon radiation (IR) injury. This study aims to: (1) determine whether EGCG can support epithelial proliferation during homeostasis; and (2) investigate what epithelial cells are protected by EGCG from IR injury. Ex vivo mouse SG were treated with EGCG from 7.5–30 µg/mL for up to 72 h. Next, SG epithelial branching morphogenesis was evaluated by bright-field microscopy, immunofluorescence, and gene expression arrays. To establish IR injury models, linear accelerator (LINAC) technologies were utilized, and radiation doses optimized. EGCG epithelial effects in these injury models were assessed using light, confocal and electron microscopy, the Griess assay, immunohistochemistry, and gene arrays. SG pretreated with EGCG 7.5 µg/mL promoted epithelial proliferation and the development of pro-acinar buds and ducts in regular homeostasis. Furthermore, EGCG increased the populations of epithelial progenitors in buds and ducts and pro-acinar cells, most probably due to its observed antioxidant activity after IR injury, which prevented epithelial apoptosis. Future studies will assess the potential for nanocarriers to increase the oral bioavailability of EGCG.