Publication: Simple in-house flow-injection capillary electrophoresis with capacitively coupled contactless conductivity method for the determination of colistin
Issued Date
2015-03-01
Resource Type
ISSN
16159314
16159306
16159306
Other identifier(s)
2-s2.0-84925349843
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Separation Science. Vol.38, No.6 (2015), 1035-1041
Suggested Citation
Patcharin Chaisuwan, Thararat Moonta, Areeporn Sangcakul, Duangjai Nacapricha, Prapin Wilairat, Kanchana Uraisin Simple in-house flow-injection capillary electrophoresis with capacitively coupled contactless conductivity method for the determination of colistin. Journal of Separation Science. Vol.38, No.6 (2015), 1035-1041. doi:10.1002/jssc.201401101 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35705
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Title
Simple in-house flow-injection capillary electrophoresis with capacitively coupled contactless conductivity method for the determination of colistin
Abstract
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. An in-house flow-injection capillary electrophoresis with capacitively coupled contactless conductivity detection method was developed for the direct measurement of colistin in pharmaceutical samples. The flow injection and capillary electrophoresis systems are connected by an acrylic interface. Capillary electrophoresis separation is achieved within 2 min using a background electrolyte solution of 5 mM 2-morpholinoethanesulfonic acid and 5 mM histidine (pH 6). The flow-injection section allows for convenient filling of the capillary and sample introduction without the use of a pressure/vacuum manifold. Capacitively coupled contactless conductivity detection is employed since colistin has no chromophore but is cationic at pH 6. Calibration curve is linear from 20 to 150 mg/L, with a correlation coefficient (r2) of 0.997. The limit of quantitation is 20 mg/L. The developed method provides precision, simplicity, and short analysis time.