Publication: Shrimp laminin receptor binds with capsid proteins of two additional shrimp RNA viruses YHV and IMNV
Issued Date
2011-07-01
Resource Type
ISSN
10959947
10504648
10504648
Other identifier(s)
2-s2.0-79957875645
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology. Vol.31, No.1 (2011), 66-72
Suggested Citation
Nattaphon Busayarat, Saengchan Senapin, Moltira Tonganunt, Kornsunee Phiwsaiya, Watcharachai Meemetta, Sasimanas Unajak, Sarawut Jitrapakdee, Chu Fang Lo, Amornrat Phongdara Shrimp laminin receptor binds with capsid proteins of two additional shrimp RNA viruses YHV and IMNV. Fish and Shellfish Immunology. Vol.31, No.1 (2011), 66-72. doi:10.1016/j.fsi.2011.03.007 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11302
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Shrimp laminin receptor binds with capsid proteins of two additional shrimp RNA viruses YHV and IMNV
Abstract
Laminin receptor (Lamr) in shrimp was previously proposed to be a potential receptor protein for Taura syndrome virus (TSV) based on yeast two-hybrid assays. Since shrimp Lamr bound to the VP1 capsid protein of TSV, we were interested to know whether capsid/envelope proteins from other shrimp viruses would also bind to Lamr. Thus, capsid/envelope encoding genes from 5 additional shrimp viruses were examined. These were Penaeus stylirostris densovirus (PstDNV), white spot syndrome virus (WSSV), infectious myonecrosis virus (IMNV), Macrobrachium rosenbergii nodavirus (MrNV), and yellow head virus (YHV). Protein interaction analysis using yeast two-hybrid assay revealed that Lamr specifically interacted with capsid/envelope proteins of RNA viruses IMNV and YHV but not MrNV and not with the capsid/envelope proteins of DNA viruses PstDNV and WSSV. In vitro pull-down assay also confirmed the interaction between Lamr and YHV gp116 envelope protein, and injection of recombinant Lamr (rLamr) protein produced in yeast cells protected shrimp against YHV in laboratory challenge tests. © 2011 Elsevier Ltd.
