Publication: The fundamental parameters of chitosan in polymer scaffolds affecting osteoblasts (MC3T3-E1)
Issued Date
2009-01-01
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ISSN
09574530
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2-s2.0-58549095429
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Materials Science: Materials in Medicine. Vol.20, No.1 (2009), 309-320
Suggested Citation
Wiroj Suphasiriroj, Pusadee Yotnuengnit, Rudee Surarit, Rath Pichyangkura The fundamental parameters of chitosan in polymer scaffolds affecting osteoblasts (MC3T3-E1). Journal of Materials Science: Materials in Medicine. Vol.20, No.1 (2009), 309-320. doi:10.1007/s10856-008-3575-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27319
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Title
The fundamental parameters of chitosan in polymer scaffolds affecting osteoblasts (MC3T3-E1)
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Abstract
The aim of this study was to investigate the degree of deacetylation (DD) and molecular weight (MW) of chitosan within chitosan-collagen scaffolds on mouse osteoblasts (MC3T3-E1). The chitosan-collagen scaffolds were fabricated by freeze-drying technique. The studies on cell attachment and proliferation, alkaline phosphatase (ALP) activity, cell morphology, and mineralized nodule formation by osteoblasts on scaffolds were investigated. No statistically significant difference was found on cell attachment, but the chitosan-collagen scaffolds with low-DD chitosan had a statistically significantly (P < 0.05) higher proliferative effect and ALP activity than those scaffolds with high-DD chitosan, regardless of molecular weight. Scanning electron images demonstrated that MC3T3-E1 cells grew well on all test scaffolds; on the contrary, mineralized nodule formation was not found. In conclusion, the DD of chitosan is a crucial factor for MC3T3-E1 cells and it should be considered in further applications for bone tissue engineering. © 2008 Springer Science+Business Media, LLC.