Publication: Anti-Acanthamoeba synergistic effect of chlorhexidine and Garcinia mangostana extract or α-mangostin against Acanthamoeba triangularis trophozoite and cyst forms
Issued Date
2021-12-01
Resource Type
ISSN
20452322
Other identifier(s)
2-s2.0-85104254376
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports. Vol.11, No.1 (2021)
Suggested Citation
Suthinee Sangkanu, Watcharapong Mitsuwan, Wilawan Mahabusarakam, Tajudeen O. Jimoh, Polrat Wilairatana, Ana Paula Girol, Ajoy K. Verma, Maria de Lourdes Pereira, Mohammed Rahmatullah, Christophe Wiart, Abolghasem Siyadatpanah, Roghayeh Norouzi, Polydor Ngoy Mutombo, Veeranoot Nissapatorn Anti-Acanthamoeba synergistic effect of chlorhexidine and Garcinia mangostana extract or α-mangostin against Acanthamoeba triangularis trophozoite and cyst forms. Scientific Reports. Vol.11, No.1 (2021). doi:10.1038/s41598-021-87381-x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/79254
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Anti-Acanthamoeba synergistic effect of chlorhexidine and Garcinia mangostana extract or α-mangostin against Acanthamoeba triangularis trophozoite and cyst forms
Other Contributor(s)
Islamic University in Uganda
The University of Nottingham Malaysia Campus
Chulalongkorn University
Walailak University
Universidade de Aveiro
Birjand University of Medical Sciences
Mahidol University
UNSW Medicine
University of Tabriz
Prince of Songkla University
Universidade Estadual Paulista "Júlio de Mesquita Filho"
Burnet Institute
National Institute of Tuberculosis and Respiratory Diseases
University of Development Alternative
The University of Nottingham Malaysia Campus
Chulalongkorn University
Walailak University
Universidade de Aveiro
Birjand University of Medical Sciences
Mahidol University
UNSW Medicine
University of Tabriz
Prince of Songkla University
Universidade Estadual Paulista "Júlio de Mesquita Filho"
Burnet Institute
National Institute of Tuberculosis and Respiratory Diseases
University of Development Alternative
Abstract
Acanthamoeba spp. can cause amoebic keratitis (AK). Chlorhexidine is effective for AK treatment as monotherapy, but with a relative failure on drug bioavailability in the deep corneal stroma. The combination of chlorhexidine and propamidine isethionate is recommended in the current AK treatment. However, the effectiveness of treatment depends on the parasite and virulence strains. This study aims to determine the potential of Garcinia mangostana pericarp extract and α-mangostin against Acanthamoeba triangularis, as well as the combination with chlorhexidine in the treatment of Acanthamoeba infection. The minimal inhibitory concentrations (MICs) of the extract and α-mangostin were assessed in trophozoites with 0.25 and 0.5 mg/mL, for cysts with 4 and 1 mg/mL, respectively. The MIC of the extract and α-mangostin inhibited the growth of A. triangularis trophozoites and cysts for up to 72 h. The extract and α-mangostin combined with chlorhexidine demonstrated good synergism, resulting in a reduction of 1/4–1/16 of the MIC. The SEM results showed that Acanthamoeba cells treated with a single drug and its combination caused damage to the cell membrane and irregular cell shapes. A good combination displayed by the extract or α-mangostin and chlorhexidine, described for the first time. Therefore, this approach is promising as an alternative method for the management of Acanthamoeba infection in the future.