Publication: Antibodies in melioidosis: The role of the indirect hemagglutination assay in evaluating patients and exposed populations
Issued Date
2018-01-01
Resource Type
ISSN
00029637
Other identifier(s)
2-s2.0-85058178683
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.99, No.6 (2018), 1378-1385
Suggested Citation
Panjaporn Chaichana, Kemajittra Jenjaroen, Premjit Amornchai, Suchintana Chumseng, Sayan Langla, Patpong Rongkard, Manutsanun Sumonwiriya, Atthanee Jeeyapant, Narisara Chantratita, Prapit Teparrukkul, Direk Limmathurotsakul, Nicholas P.J. Day, Vanaporn Wuthiekanun, Susanna J. Dunachie Antibodies in melioidosis: The role of the indirect hemagglutination assay in evaluating patients and exposed populations. American Journal of Tropical Medicine and Hygiene. Vol.99, No.6 (2018), 1378-1385. doi:10.4269/ajtmh.17-0998 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/46064
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Antibodies in melioidosis: The role of the indirect hemagglutination assay in evaluating patients and exposed populations
Other Contributor(s)
Abstract
Copyright © 2018 by The American Society of Tropical Medicine and Hygiene. Melioidosis is a major neglected tropical disease with high mortality, caused by the Gram-negative bacterium Burkholderia pseudomallei (Bp). Microbiological culture remains the gold standard for diagnosis, but a simpler and more readily available test such as an antibody assay is highly desirable. In this study, we conducted a serological survey of blood donors (n = 1,060) and adult melioidosis patients (n = 200) in northeast Thailand to measure the antibody response to Bp using the indirect hemagglutination assay (IHA). We found that 38% of healthy adults (aged 17–59 years) have seropositivity (IHA titer 3 1:80). The seropositivity in healthy blood donors was associated with having a declared occupation of rice farmer and with residence in a nonurban area, but not with gender or age. In the melioidosis cohort, the seropositivity rate was higher in adult patients aged between 18 and 45 years (90%, 37/41) compared with those aged 3 45 years (68%, 108/159, P = 0.004). The seropositivity rate was significantly higher in people with diabetes (P = 0.008). Seropositivity was associated with decreased mortality on univariable analysis (P = 0.005), but not on multivariable analysis when adjusted for age, diabetes status, preexisting renal disease, and neutrophil count. This study confirms the presence of high background antibodies in an endemic region and demonstrates the limitations of using IHA during acute melioidosis in this population.
