Publication: Early effects of rhodomyrtone on membrane integrity in methicillin-resistant staphylococcus aureus
Issued Date
2018-09-01
Resource Type
ISSN
19318448
10766294
10766294
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2-s2.0-85053164868
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Mahidol University
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SCOPUS
Bibliographic Citation
Microbial Drug Resistance. Vol.24, No.7 (2018), 882-889
Suggested Citation
Wipawadee Sianglum, Dennapa Saeloh, Pongsri Tongtawe, Natthakul Wootipoom, Nitaya Indrawattana, Supayang Piyawan Voravuthikunchai Early effects of rhodomyrtone on membrane integrity in methicillin-resistant staphylococcus aureus. Microbial Drug Resistance. Vol.24, No.7 (2018), 882-889. doi:10.1089/mdr.2016.0294 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45980
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Title
Early effects of rhodomyrtone on membrane integrity in methicillin-resistant staphylococcus aureus
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Abstract
© 2018 Mary Ann Liebert, Inc. Strong evidence of high potency of rhodomyrtone as a promising antibacterial agent against pathogenic gram-positive bacteria has been clearly demonstrated in our previous work. The aim of this study was to provide insight into early action of rhodomyrtone, an acylphloroglucinol, on membrane damage in multidrug-resistant methicillin-resistant Staphylococcus aureus (MRSA). Early effects of rhodomyrtone on the bacterial membrane integrity were detected in a time-course study. Flow cytometry revealed a reduction in green fluorescent emission and increase in uptake of propidium iodide in rhodomyrtone-treated bacterial cells in a concentration- A nd time-dependent manner. Disruption of cytoplasmic membrane was further monitored by measuring cellular adenosine triphosphate (ATP) and potassium ion (K+). Leakage of both ATP and K+ and significant decrease in intracellular ATP in MRSA were observed following treatment. Pronounced changes in the bacterial ultrastructure and morphology were confirmed by transmission electron microscopy and scanning electron microscopy. Bacterial cell disruption, holes in cell surface, and bulge formations were noted in rhodomyrtone-treated cells. In this study, we provided relevant data to clarify that rhodomyrtone is a bacterial cell membrane-damaging agent. A possible early effect of this novel compound involves bacterial membrane disruption.