Publication: Thermal response of in vivo human skin to fractional radiofrequency microneedle device
Issued Date
2016-01-01
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23146141
23146133
23146133
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2-s2.0-84973345039
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Mahidol University
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SCOPUS
Bibliographic Citation
BioMed Research International. Vol.2016, (2016)
Suggested Citation
Woraphong Manuskiatti, Penvadee Pattanaprichakul, Siriluk Inthasotti, Panitta Sitthinamsuwan, Suchanan Hanamornroongruang, Rungsima Wanitphakdeedecha, Sorawuth Chu-Ongsakol Thermal response of in vivo human skin to fractional radiofrequency microneedle device. BioMed Research International. Vol.2016, (2016). doi:10.1155/2016/6939018 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/43125
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Title
Thermal response of in vivo human skin to fractional radiofrequency microneedle device
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Abstract
© 2016 Woraphong Manuskiatti et al. Background. Fractional radiofrequency microneedle system (FRMS) is a novel fractional skin resurfacing system. Data on thermal response to this fractional resurfacing technique is limited. Objectives. To investigate histologic response of in vivo human skin to varying energy settings and pulse stacking of a FRMS in dark-skinned subjects. Methods. Two female volunteers who were scheduled for abdominoplasty received treatment with a FRMS with varying energy settings at 6 time periods including 3 months, 1 month, 1 week, 3 days, 1 day, and the time immediately before abdominoplasty. Biopsy specimens were analyzed using hematoxylin and eosin (H&E), Verhoeff-Van Gieson (VVG), colloidal iron, and Fontana-Masson stain. Immunohistochemical study was performed by using Heat Shock Protein 70 (HSP70) antibody and collagen III monoclonal antibody. Results. The average depth of radiofrequency thermal zone (RFTZ) ranged from 100 to 300 μm, correlating with energy levels. Columns of cell necrosis and collagen denaturation followed by inflammatory response were initially demonstrated, with subsequent increasing of mucin at 1 and 3 months after treatment. Immunohistochemical study showed positive stain with HSP70. Conclusion. A single treatment with a FRMS using appropriate energy setting induces neocollagenesis. This wound healing response may serve as a mean to improve the appearance of photodamaged skin and atrophic scars.