Publication: Development of genotype-independent regeneration system for transformation of rice (Oryza sativa ssp. indica)
Issued Date
2007-03-01
Resource Type
ISSN
09189440
Other identifier(s)
2-s2.0-33947250119
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Plant Research. Vol.120, No.2 (2007), 237-245
Suggested Citation
Nimnara Yookongkaew, Methinee Srivatanakul, Jarunya Narangajavana Development of genotype-independent regeneration system for transformation of rice (Oryza sativa ssp. indica). Journal of Plant Research. Vol.120, No.2 (2007), 237-245. doi:10.1007/s10265-006-0046-z Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24025
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Development of genotype-independent regeneration system for transformation of rice (Oryza sativa ssp. indica)
Other Contributor(s)
Abstract
Rice (Oryza sativa ssp. indica) is an important economic crop in many countries. Although a variety of conventional methods have been developed to improve this plant, manipulation by genetic engineering is still complicated. We have established a system of multiple shoot regeneration from rice shoot apical meristem. By use of MS medium containing 4 mgn L-1thidiazuron (TDZ) multiple shoots were successfully developed directly from the meristem without an intervening callus stage. All rice cultivars tested responded well on the medium and regenerated to plantlets that were readily transferred to soil within 5-8 weeks. The tissue culture system was suitable for Agrobacterium-mediated transformation and different factors affecting transformation efficiency were investigated. Agrobacterium strain EHA105 containing the plasmid pCAMBIA1301 was used. The lowest concentration of hygromycin B in combined with either 250 mg L-1carbenicillin or 250 mg L-1cefotaxime to kill the rice shoot apical meristem was 50 mg L-1and carbenicillin was more effective than cefotaxime. Two-hundred micromolar acetosyringone had no effect on the efficiency of transient expression. Sonication of rice shoot apical meristem for 10 s during bacterial immersion increased transient GUS expression in three-day co-cultivated seedlings. The gus gene was found to be integrated into the genome of the T0transformant plantlets. © The Botanical Society of Japan and Springer 2007.