Publication: Nucleophosmin may act as an alarmin: Implications for severe sepsis
Issued Date
2009-09-01
Resource Type
ISSN
07415400
Other identifier(s)
2-s2.0-70149116131
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Leukocyte Biology. Vol.86, No.3 (2009), 645-653
Suggested Citation
Yuko Nawa, Ko Ichi Kawahara, Salunya Tancharoen, Xiaojie Meng, Hisayo Sameshima, Takashi Ito, Yoshiki Masuda, Hitoshi Imaizumi, Teruto Hashiguchi, Ikuro Maruyama Nucleophosmin may act as an alarmin: Implications for severe sepsis. Journal of Leukocyte Biology. Vol.86, No.3 (2009), 645-653. doi:10.1189/jlb.1008644 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27150
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Title
Nucleophosmin may act as an alarmin: Implications for severe sepsis
Abstract
NPM is a major nucleolar multifunctional protein involved in ribosome biogenesis, centrosome duplication, cell-cycle progression, apoptosis, cell differentiation, and sensing cellular stress. Alarmins are endogenous molecules released from activated cells and/or dying cells, which activate the immune system and cause severe damage to cells and tissue organs. In the present work, stimulation of cells with the alarmin-inducible molecule endotoxin, for 16 h, resulted in NPM release into the culture supernatants of RAW264.7 cells, a murine macrophage cell line. Extracellular NPM was detected in the ascites of the CLP model. NPM was translocated into the cytoplasm from the nucleus in LPS -stimulated RAW264.7 cells; furthermore, NPM was detected in the cytosols of infiltrated macrophages in the CLP model. rNPM induced release of proinflammatory cytokines, TNF-α, IL-6, and MCP-1, from RAW264.7 cells and increased the expression level of ICAM-1 in HUVECs. NPM induced the phosphorylation of MAPKs in RAW264.7 cells. Our data indicate that NPM may have potent biological activities that contribute to systemic inflammation. Further investigations of the role of NPM may lead to new therapies for patients with septic shock or other inflammatory diseases. © Society for Leukocyte Biology.