Publication: A sensitive method for the determination of tranexamic acid in human serum by liquid chromatography with tandem mass spectrometer
Issued Date
2014-02-11
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ISSN
01252526
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2-s2.0-84893496358
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Mahidol University
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SCOPUS
Bibliographic Citation
Chiang Mai Journal of Science. Vol.41, No.1 (2014), 156-165
Suggested Citation
Paisarn Jittorntrum, Saowanee Kajanachumpol, Duangkamol Viroonudomphol, Prapin Wilairat A sensitive method for the determination of tranexamic acid in human serum by liquid chromatography with tandem mass spectrometer. Chiang Mai Journal of Science. Vol.41, No.1 (2014), 156-165. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33303
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Title
A sensitive method for the determination of tranexamic acid in human serum by liquid chromatography with tandem mass spectrometer
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Abstract
Tranexamic acid (TA) is a synthetic lysine analog used for the management of bleeding disorders. In this study, we developed and validated a method for the determination of TA in human serum by liquid chromatography with Q-trap mass spectrometer. Serum sample (100 μL) was deproteinated with perchloric acid, and after pH adjustment, chromatographic separation was performed on a C18 column and isocratically eluted using a mobile phase consisting of ammonium acetate buffer (pH 3.8) /acetonitrile (95:5, v/v) at a flow rate of 200 μL /min. The total run time was 5 minutes. Detection and quantitation were performed with the mass spectrometer using multiple reaction monitoring mode with the ion transition m/z 158.1 to m/z 95.1 for TA and m/z 144.0 to m/z 81.1 for the internal standard (cis-4-aminocyclohexanecarboxylic acid). The results were linear over the concentration range of 0.1-100 μg/mL of TA, with limit of quantitation of 0.03 μg/mL. The intra-day and inter-day assay coefficient of variations for serum were less than 1.8% and 2.1%, respectively, and the recovery of added standard TA was 92.5 to 99.3%. In conclusion; a simple and sensitive LC-MS/MS method has been developed for the determination of TA in human serum. The method showed excellent linearity, sensitivity, recovery and precision. This method is suitable for clinical pharmacokinetic studies.