Publication: A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border
Issued Date
2011-01-01
Resource Type
ISSN
00359203
Other identifier(s)
2-s2.0-78649686678
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Mahidol University
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SCOPUS
Bibliographic Citation
Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.105, No.1 (2011), 32-37
Suggested Citation
Wanitda Watthanaworawit, Paul Turner, Claudia L. Turner, Ampai Tanganuchitcharnchai, Richard G. Jarman, Stuart D. Blacksell, François H. Nosten A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border. Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.105, No.1 (2011), 32-37. doi:10.1016/j.trstmh.2010.09.007 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12101
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Title
A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border
Abstract
Clinically useful diagnostic tests of dengue virus infection are lacking. We prospectively evaluated the performance of real-time reverse transcriptase (rRT)-PCR, NS-1 antigen and IgM antibody tests to confirm dengue virus infection in acute blood specimens from 162 patients presenting with undifferentiated febrile illness compatible with dengue infection. rRT-PCR was the most sensitive test (89%) and potentially could be used as a single test for confirmation of dengue infection. NS-1 antigen and IgM antibody were not sufficiently sensitive to be used as a single confirmatory test with sensitivities of 54% and 17% respectively. The specificities of rRT-PCR, NS-1 antigen and IgM antibody tests were 96%, 100% and 88% respectively. Combining NS-1 and rRT-PCR or the combination of all three tests resulted in the highest sensitivity (93%) but specificities dropped to 96% and 83% respectively. We conclude that at least the combination of two tests, either agent detection (rRT-PCR) or antigen detection (NS-1) plus IgM antibody detection should be used for laboratory confirmation of dengue infection. © 2010 Royal Society of Tropical Medicine and Hygiene.