Publication: Role of Plasmodium falciparum chloroquine resistance transporter and multidrug resistance 1 genes on in vitro chloroquine resistance in isolates of Plasmodium falciparum from Thailand
Issued Date
2011-10-01
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00029637
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2-s2.0-80053897206
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.85, No.4 (2011), 606-611
Suggested Citation
Chavachol Setthaudom, Peerapan Tan-Ariya, Naruemon Sitthichot, Rommanee Khositnithikul, Nantana Suwandittakul, Saovanee Leelayoova, Mathirut Mungthin Role of Plasmodium falciparum chloroquine resistance transporter and multidrug resistance 1 genes on in vitro chloroquine resistance in isolates of Plasmodium falciparum from Thailand. American Journal of Tropical Medicine and Hygiene. Vol.85, No.4 (2011), 606-611. doi:10.4269/ajtmh.2011.11-0108 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11983
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Title
Role of Plasmodium falciparum chloroquine resistance transporter and multidrug resistance 1 genes on in vitro chloroquine resistance in isolates of Plasmodium falciparum from Thailand
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Abstract
Resistance to chloroquine is a public health problem worldwide. Polymorphisms of the Plasmodium falciparum chloroquine resistance transporter ( pfcrt ) and P. falciparum multidrug resistance 1 ( pfmdr1 ) genes have been linked to chloroquine resistance. Although the K76T mutation in the pfcrt gene has been shown to be a key determinant in chloroquine resistance, evidence suggests that the pfmdr1 gene could modulate the level of chloroquine resistance. However, few studies of field isolates could identify the interactive role of these two genes in chloroquine resistance. Thus, we evaluated the influence of pfcrt and pfmdr1 polymorphisms on in vitro chloroquine sensitivity in 89 adapted isolates of P. falciparum from Thailand. We found that 87 of 89 isolates contained the CVIET haplotype of the pfcrt gene. Two additional mutations in the pfcrt gene were identified, i.e., K6Q and H97L. For the pfmdr1 polymorphisms, the 184F allele was common in the parasites isolated along the Thailand-Cambodia border, and those isolated along the Thailand-Myanmar border contained higher copy numbers. Our results indicate that the additional mutations, in particular H97L in the pfcrt gene and Y184F in the pfmdr1 gene and its copy number, influence the level of chloroquine resistance. Copyright © 2011 by The American Society of Tropical Medicine and Hygiene.