Publication: Antimicrobial Property of Hydrocolloid Impression Material Incorporated with Silver Nanoparticles Against Staphylococcus Aureus
Issued Date
2017-02-09
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ISSN
2261236X
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2-s2.0-85013391460
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Mahidol University
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SCOPUS
Bibliographic Citation
MATEC Web of Conferences. Vol.95, (2017)
Suggested Citation
Norbu Penden Wangchuk, Passiri Nisalak, Boonyanit Thaweboon, Sroisiri Thawboon, Pornrachanee Sawaengkit Antimicrobial Property of Hydrocolloid Impression Material Incorporated with Silver Nanoparticles Against Staphylococcus Aureus. MATEC Web of Conferences. Vol.95, (2017). doi:10.1051/matecconf/20179501001 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42227
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Title
Antimicrobial Property of Hydrocolloid Impression Material Incorporated with Silver Nanoparticles Against Staphylococcus Aureus
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Abstract
© The Authors, published by EDP Sciences, 2017. Dental impressions can easily become contaminated with patient's blood and saliva which are capable of transmitting infectious diseases to dental personnel. The addition of antimicrobial agents into impression materials could be effective in reducing the chances of cross-infection. Silver nanoparticles have been applied in dentistry as a potent antimicrobial agent. This study aims to evaluate the in vitro antimicrobial efficacy of silver nanoparticles incorporated to irreversible hydrocolloid impression material against Staphylococcus aureus. Silver nanoparticles (AgZrPO4, National Direct Network Company, Thailand) at concentrations of 0.25%, 0.50%, 1.00% and 1.50% w/w were added to powder of impression materials (Kromopan, Lascod, Ilaty). Impression material samples were prepared on sterile plate in accordance with manufacturer's instruction. After setting, a 100 microliter of S. aureus ATCC6538 suspension (106cells/mL) were inoculated on the surface of the impression sample and left for 10 minutes. The amount of S. aureus on the surface was quantified using imprint technique on Mannitol Salt agar. Impression materials incorporated with AgZrPO4showed antimicrobial property against S. aureus (up to 95% reduction) compared with control (impression material without AgZrPO4). Even though the mechanism of antimicrobial action was not clearly understood, AgZrPO4incorporated to impression material was demonstrated to possess an inhibitory effect against pathogenic bacteria. Further studies are needed to investigate physical properties of the material and the clinical usage.