Publication: Transport of the Thyroid Hormone Carrier Protein Transthyretin into Human Epidermoid Cells
Issued Date
2019-01-01
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ISSN
15324206
07435800
07435800
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2-s2.0-85075540082
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Mahidol University
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SCOPUS
Bibliographic Citation
Endocrine Research. (2019)
Suggested Citation
Vai Hong Fong, Shaun Wong, Ponrutsami Jintaridhi, Amandio Vieira Transport of the Thyroid Hormone Carrier Protein Transthyretin into Human Epidermoid Cells. Endocrine Research. (2019). doi:10.1080/07435800.2019.1694538 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50343
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Title
Transport of the Thyroid Hormone Carrier Protein Transthyretin into Human Epidermoid Cells
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Abstract
© 2019, © 2019 Taylor & Francis Group, LLC. Purpose: Transthyretin (TTR) is a protein with a growing number of biological functions in addition to its well-established binding and circulatory transport of thyroxine, and indirect retinoid transport through interaction with retinol-binding protein. Misfolded and aggregated wild-type and mutant TTRs are involved in amyloid diseases. Several aspects of TTR pathology and physiology remain poorly understood. Receptor-mediated cellular transport of TTR has been described in a few cell types; and such studies suggest the possibility of different TTR receptors and endocytic pathways. Our main objective was to further understand the endocytic pathways for TTR. Methods: In the current study, analyses of TTR endocytic transport were performed in the human A431 cell line. The results of TTR uptake were compared with those of the iron-carrier protein transferrin (Tf, a common stardard for endocytosis studies) in the same cell types. Results: A comparison of TTR and Tf endocytosis suggested similar early, 5–10 min, accumulation kinetics. But at a later time, 30 min, TTR accumulation was 20-30% lower than that of Tf (p < .05), a result that suggests different post-endocytic fates for these two ligands. Through the use of multiple endocytosis inhibitors, biochemical evidence is provided for an internalization pathway that differs from the clathrin-mediated endocytosis of Tf. Conclusions: These results for A431 cells are compared with others reported for different cell types; and it is suggested that this same hormone carrier protein can transit into cells through multiple endocytic pathways.