Publication: Engineering of Corynebacterium glutamicum as a prototrophic pyruvate-producing strain: Characterization of a ramA-deficient mutant and its application for metabolic engineering
Issued Date
2019-01-01
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ISSN
13476947
09168451
09168451
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2-s2.0-85060382575
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Mahidol University
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SCOPUS
Bibliographic Citation
Bioscience, Biotechnology and Biochemistry. Vol.83, No.2 (2019), 372-380
Suggested Citation
Naoya Kataoka, Alisa S. Vangnai, Thunyarat Pongtharangkul, Toshiharu Yakushi, Masaru Wada, Atsushi Yokota, Kazunobu Matsushita Engineering of Corynebacterium glutamicum as a prototrophic pyruvate-producing strain: Characterization of a ramA-deficient mutant and its application for metabolic engineering. Bioscience, Biotechnology and Biochemistry. Vol.83, No.2 (2019), 372-380. doi:10.1080/09168451.2018.1527211 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50383
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Title
Engineering of Corynebacterium glutamicum as a prototrophic pyruvate-producing strain: Characterization of a ramA-deficient mutant and its application for metabolic engineering
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Abstract
© 2018 Japan Society for Bioscience, Biotechnology, and Agrochemistry. To construct a prototrophic Corynebacterium glutamicum strain that efficiently produces pyruvate from glucose, the effects of inactivating RamA, a global regulator responsible for activating the oxidative tricarboxylic acid (TCA) cycle, on glucose metabolism were investigated. ΔramA showed an increased specific glucose consumption rate, decreased growth, comparable pyruvate production, higher formation of lactate and acetate, and lower accumulation of succinate and 2-oxoglutarate compared to the wild type. A significant decrease in pyruvate dehydrogenase complex activity was observed for ΔramA, indicating reduced carbon flow to the TCA cycle in ΔramA. To create an efficient pyruvate producer, the ramA gene was deleted in a strain lacking the genes involved in all known lactate- and acetate-producing pathways. The resulting mutant produced 161 mM pyruvate from 222 mM glucose, which was significantly higher than that of the parent (89.3 mM; 1.80-fold).