Publication: Methane Production from the Co-digestion of Algal Biomass with Crude Glycerol by Anaerobic Mixed Cultures
Issued Date
2018-01-01
ISSN
1877265X
18772641
18772641
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2-s2.0-85058639358
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Mahidol University
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SCOPUS
Bibliographic Citation
Waste and Biomass Valorization. (2018)
Suggested Citation
Sureewan Sittijunda, Alissara Reungsang Methane Production from the Co-digestion of Algal Biomass with Crude Glycerol by Anaerobic Mixed Cultures. Waste and Biomass Valorization. (2018). doi:10.1007/s12649-018-0542-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45778
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Title
Methane Production from the Co-digestion of Algal Biomass with Crude Glycerol by Anaerobic Mixed Cultures
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Abstract
© 2018, Springer Nature B.V. The optimization of factors affecting methane production from the co-digestion of algal biomass with crude glycerol was investigated using response surface methodology with a central composite design. The investigated parameters included algal biomass, crude glycerol and inoculum concentration. The inoculum and algal biomass concentration had a significant individual effect on the methane production (p ≤ 0.05). The interactive effect on methane production was found to be between algal biomass and crude glycerol (p ≤ 0.05). The optimal conditions were 20.02 g-VS/L of crude glycerol, 9.76 g-VS/L of inoculum concentration and 5.50 g-VS/L of algal biomass. Under optimum conditions, the maximum methane production of 58.88 mL-CH4/L was obtained. The difference between the observed methane production (58.88 mL-CH4/L) and the predicted methane (68.94 mL-CH4/L) was 14.59%. Under the optimum conditions, the energy production of 92.47 J/g-VSadded was obtained. The polymerase chain reaction-denaturing gradient gel electrophoresis analysis indicated that the methane producers present in the fermentation broth were Methanosarcina sp., Methanoregula sp., Methanospirillum sp. and Methanoculleus sp.