Publication: Comparison of pouzolzia indica methanolic extract and virkon® against cysts of acanthamoeba SPP
Issued Date
2010-07-01
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ISSN
01251562
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2-s2.0-79952114378
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.41, No.4 (2010), 776-784
Suggested Citation
Kosol Roongruangchai, Tanawan Kummalue, Tichaporn Sookkua, Jantima Roongruangchai Comparison of pouzolzia indica methanolic extract and virkon® against cysts of acanthamoeba SPP. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.41, No.4 (2010), 776-784. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/29602
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Title
Comparison of pouzolzia indica methanolic extract and virkon® against cysts of acanthamoeba SPP
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Abstract
The present study was conducted to investigate the morphological and structural changes of Acanthamoeba cysts after being treated with various concentrations of Pouzolzia indica methanolic extract fraction 3 (methanol eluted) and Virkon® solution. Changes in the Acanthamoeba cysts were detected by light microscopy, scanning electron microscopy and transmission electron microscopy. The results show Acanthamoeba cysts were killed by Pouzolzia indica methanolic extract fraction 3 at a concentration of 1:8 and by Virkon® solution at a concentration of 0.25%, with a minimal cysticidal concentration (MCC) by 24 hours. Both agents caused similar structural damage to Acanthamoeba cysts in the same sequence. Step by step structural alterations occurred within the cyst. First, the cyst shrank, collapsed and had clumping of cytoplasmic stuctures inside the cyst walls. Second, the cysts began to bulge, swell, have a decrease in wrinkles in the cyst walls and spill the cytoplasmic contents into the environment. Finally, the cyst walls broke into small pieces. This study may be beneficial to compare with future studies of pharmaceutical agents against Acanthamoeba keratitis.
