Publication: Improved yeast-screening assay for phytoestrogens
Issued Date
2021-06-01
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ISSN
13476947
09168451
09168451
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2-s2.0-85107090923
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Mahidol University
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SCOPUS
Bibliographic Citation
Bioscience, Biotechnology and Biochemistry. Vol.85, No.6 (2021), 1422-1432
Suggested Citation
Natthida Sophon, Choladda Phiwpesh, Sieng Darith, Wichai Cherdshewasart, Chuenchit Boonchird Improved yeast-screening assay for phytoestrogens. Bioscience, Biotechnology and Biochemistry. Vol.85, No.6 (2021), 1422-1432. doi:10.1093/bbb/zbab051 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76163
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Title
Improved yeast-screening assay for phytoestrogens
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Abstract
A yeast estrogen screening (YES) assay was improved to increase sensitivity for detection of phytoestrogens. New yeast strains minus one or the other of transporters Pdr5 or Snq2 and harboring yEGFP as a reporter gene were developed. The new strains showed 2-100-fold improvement in sensitivity for detection of standard estrogens and antiestrogens. In addition, the assay time (1 h) using the newly developed strains was shorter than that (4 h) previously reported. Furthermore, the snq2-minus strains were most effective for detection of estrogenic activity while the pdr5-minus strains were most effective for detection of antiestrogenic activity. The efficacy of the new methods was evaluated and confirmed by testing with 23 Thai medicinal plant species. The new strains were also tested for detection of xenoestrogens. The results revealed that the newly developed YES methods were specific and rapid and suitable for simple high-throughput screening or detection of estrogen-like compounds.