Publication: Vascular leakage in severe dengue virus infections: A potential role for the nonstructural viral protein NS1 and complement
Issued Date
2006-04-15
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ISSN
00221899
DOI
Other identifier(s)
2-s2.0-33645798035
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Infectious Diseases. Vol.193, No.8 (2006), 1078-1088
Suggested Citation
Panisadee Avirutnan, Nuntaya Punyadee, Sansanee Noisakran, Chulaluk Komoltri, Somchai Thiemmeca, Kusuma Auethavornanan, Aroonroong Jairungsri, Rattiyaporn Kanlaya, Nattaya Tangthawornchaikul, Chunya Puttikhunt, Sa Nga Pattanakitsakul, Pa Thai Yenchitsomanus, Juthathip Mongkolsapaya, Watchara Kasinrerk, Nopporn Sittisombut, Matthias Husmann, Maria Blettner, Sirijitt Vasanawathana, Sucharit Bhakdi, Prida Malasit Vascular leakage in severe dengue virus infections: A potential role for the nonstructural viral protein NS1 and complement. Journal of Infectious Diseases. Vol.193, No.8 (2006), 1078-1088. doi:10.1086/500949 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23340
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Title
Vascular leakage in severe dengue virus infections: A potential role for the nonstructural viral protein NS1 and complement
Author(s)
Panisadee Avirutnan
Nuntaya Punyadee
Sansanee Noisakran
Chulaluk Komoltri
Somchai Thiemmeca
Kusuma Auethavornanan
Aroonroong Jairungsri
Rattiyaporn Kanlaya
Nattaya Tangthawornchaikul
Chunya Puttikhunt
Sa Nga Pattanakitsakul
Pa Thai Yenchitsomanus
Juthathip Mongkolsapaya
Watchara Kasinrerk
Nopporn Sittisombut
Matthias Husmann
Maria Blettner
Sirijitt Vasanawathana
Sucharit Bhakdi
Prida Malasit
Nuntaya Punyadee
Sansanee Noisakran
Chulaluk Komoltri
Somchai Thiemmeca
Kusuma Auethavornanan
Aroonroong Jairungsri
Rattiyaporn Kanlaya
Nattaya Tangthawornchaikul
Chunya Puttikhunt
Sa Nga Pattanakitsakul
Pa Thai Yenchitsomanus
Juthathip Mongkolsapaya
Watchara Kasinrerk
Nopporn Sittisombut
Matthias Husmann
Maria Blettner
Sirijitt Vasanawathana
Sucharit Bhakdi
Prida Malasit
Abstract
Background. Vascular leakage and shock are the major causes of death in patients with dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Thirty years ago, complement activation was proposed to be a key underlying event, but the cause of complement activation has remained unknown. Methods. The major nonstructural dengue virus (DV) protein NS1 was tested for its capacity to activate human complement in its membrane-associated and soluble forms. Plasma samples from 163 patients with DV infection and from 19 patients with other febrile illnesses were prospectively analyzed for viral load and for levels of NS1 and complement-activation products. Blood and pleural fluids from 9 patients with DSS were also analyzed. Results. Soluble NS1 activated complement to completion, and activation was enhanced by polyclonal and monoclonal antibodies against NS1. Complement was also activated by cell-associated NS1 in the presence of specific antibodies. Plasma levels of NS1 and terminal SC5b-9 complexes correlated with disease severity. Large amounts of NS1, complement anaphylatoxin C5a, and the terminal complement complex SC5b-9 were present in pleural fluids from patients with DSS. Conclusions. Complement activation mediated by NS1 leads to local and systemic generation of anaphylatoxins and SC5b-9, which may contribute to the pathogenesis of the vascular leakage that occurs in patients with DHF/DSS. © 2006 by the Infectious Diseases Society of America All rights reserved.