Analysis of growth phase regulated KatA and CatE and their physiological roles in determining hydrogen peroxide resistance in Agrobacterium tumefaciens

Abstract

Agrobacterium tumefaciens possesses two catalases, a bifunctional catalase-peroxidase, KatA and a homologue of a growth phase regulated monofunctional catalase, CatE. In stationary phase cultures and in cultures entering stationary phase, total catalase activity increased 2-fold while peroxidase activity declined. katA and catE were found to be independently regulated in a growth phase dependent manner. KatA levels were highest during exponential phase and declined as cells entered stationary phase, while CatE was detectable at early exponential phase and increased during stationary phase. Only small increases in H2O2resistance levels were detected as cells entering stationary phase. The katA mutant was more sensitive to H2O2than the parental strain during both exponential and stationary phase. Inactivation of catE alone did not significantly change the level of H2O2resistance. However, the katA catE double mutant was more sensitive to H2O2during both exponential and stationary phase than either of the single catalase mutants. The data indicated that KatA plays the primary role and CatE acts synergistically in protecting A. tumefaciens from H2O2toxicity during all phases of growth. Catalase-peroxidase activity (KatA) was required for full H2O2resistance. The expression patterns of the two catalases in A. tumefaciens reflect their physiological roles in the protection against H2O2toxicity, which are different from other bacteria. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.