Publication: In vitro HIV type 1 reverse transcriptase inhibitory activities of Thai medicinal plants and Canna indica L. rhizomes
Issued Date
2005-10-03
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ISSN
03788741
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2-s2.0-24644501318
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Ethnopharmacology. Vol.101, No.1-3 (2005), 84-89
Suggested Citation
Warunya Woradulayapinij, Noppamas Soonthornchareonnon, Chanpen Wiwat In vitro HIV type 1 reverse transcriptase inhibitory activities of Thai medicinal plants and Canna indica L. rhizomes. Journal of Ethnopharmacology. Vol.101, No.1-3 (2005), 84-89. doi:10.1016/j.jep.2005.03.030 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17158
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Title
In vitro HIV type 1 reverse transcriptase inhibitory activities of Thai medicinal plants and Canna indica L. rhizomes
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Abstract
Water and 80% ethanol extracts of 20 Thai medicinal plants used to treat AIDS were tested for their HIV type 1 reverse transcriptase inhibitory activity. The water extracts of Ipomoea carnea subsp. fistulosa (aerial parts), Vitex glabrata (branch), Vitex trifolia (aerial part), Vitex negundo (aerial part), Canna indica (rhizome), and Justicia gendarussa (aerial part) showed HIV-1 RT inhibition ratio (% IR) higher than 90% at a 200 μg/ml concentration. The water extract of Canna indica rhizomes (IC5022.56 μg/ml) was selected for further study, i.e. for its HIV-1 RT inhibition activity and the purification and characterization of the active proteins. Proteins in water extract were fractionated by ammonium sulfate precipitation and separated by sodium dodecyl sulfate acrylamide gel electrophoresis (SDS-PAGE), yielding two proteins, Cip31 (31 kDa) and Cip14 (14 kDa) with IC50of 17.41 and 19.25 μg/ml and isoelectric point (pI) of 3.5 and 6.35, respectively. Both proteins showed significant HIV-1 RT inhibition. © 2005 Elsevier Ireland Ltd. All rights reserved.